Extracts of Phlomoides umbrosa Turczaninow alleviate allergic airway inflammation in lipopolysaccharide-stimulated RAW 264.7 cells and ovalbumin-induced hyper-responsiveness mouse model

• Published in: Food science and biotechnology Vol. 33; no. 11; pp. 2611 - 2621
• Main Authors: Jang, Se-Won, Jo, Ha-Neul, Han, Yoon-Young, Lee, Jae-Sun, Kim, Bo-Mi, Kim, Ji-Min, Lee, Yong-Wook, Park, Chan-Sung, Lee, Dae-Young, Kim, Kwan-Woo, Lee, Young-Seob
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01.08.2024; Springer Nature B.V; 한국식품과학회
• Subjects: Allergic rhinitis; Anti-inflammatory agents; antioxidant enzymes; Antioxidants; bone formation; Chemistry; Chemistry and Materials Science; Cytokines; Enzymatic activity; Enzyme activity; Food Science; Herbal medicine; histopathology; inducible nitric oxide synthase; Inflammation; Kinases; Lipopolysaccharides; lungs; Lymphocytes T; MAP kinase; Medicinal plants; mice; mitogen-activated protein kinase; mucus; NF-κB protein; Nitric oxide; Nitric-oxide synthase; Nutrition; Ovalbumin; Phlomis; Phlomoides umbrosa; protein synthesis; Proteins; Research Article; Respiratory tract; Respiratory tract diseases; Rhinitis; Tensin; 식품과학; Respiratory tract; Airway inflammation; Hyper-immune response; Allergic rhinitis; Turcz; Phlomoides umbrosa Turcz
• ISSN: 1226-7708; 2092-6456; 2092-6456
• DOI: 10.1007/s10068-024-01521-3

Phlomoides umbrosa Turczaninow (PU), a traditional Korean medicinal herb, exhibits osteogenic and anti-inflammatory effects. This research explored the effect of PU extracts on hyperimmune responses within the respiratory tract using lipopolysaccharide-stimulated RAW 264.7 cells and an ovalbumin-induced hyper-responsiveness model. The inflammatory cytokines, protein expression linked to airway inflammation, antioxidant enzyme activity, histopathological observation, and expectorant activity were measured. The results revealed that PU treatment led to a concentration-dependent reduction in Th2 cytokines and the expression of nuclear factor (NF)-κB, phosphatase-tensin homolog, mitogen-activated protein kinase (MAPK), and inducible nitric oxide synthase (iNOS). Simultaneously, antioxidant enzyme activity increased. Furthermore, PU exhibited substantial enhancements in lung tissue condition and expectorant activity relative to the allergic rhinitis-induced group. These findings indicate the potential of PU to mitigate airway inflammation and excessive mucus production by suppressing NF-κB, MAPK, and iNOS pathways. Consequently, PU emerges as a promising anti-inflammatory agent for respiratory tract applications.