Identification of a major immunogenic region of equine herpesvirus-1 glycoprotein E and its application to enzyme-linked immunosorbent assay

• Published in: Veterinary microbiology Vol. 164; no. 1-2; pp. 18 - 26
• Main Authors: Andoh, Kiyohiko, Takasugi, Maaya, Mahmoud, Hassan Y.A.H., Hattori, Shiho, Terada, Yutaka, Noguchi, Keita, Shimoda, Hiroshi, Bannai, Hiroshi, Tsujimura, Koji, Matsumura, Tomio, Kondo, Takashi, Maeda, Ken
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 31.05.2013
• Subjects: amino acids; Animals; antibodies; diagnosis; ELISA; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - veterinary; Epitope; Epitopes, B-Lymphocyte; Epitopes, B-Lymphocyte - isolation & purification; Equid alphaherpesvirus 1; Equine herpesvirus; Escherichia coli; genes; glutathione transferase; Glycoprotein E; Glycoprotein G; glycoproteins; Herpesviridae Infections; Herpesviridae Infections - diagnosis; Herpesviridae Infections - veterinary; Herpesvirus 1, Equid; Herpesvirus 4, Equid; Horse Diseases; Horse Diseases - diagnosis; Horses; isolation & purification; Japan; live vaccines; synthetic peptides; veterinary; Viral Envelope Proteins; Viral Envelope Proteins - isolation & purification; viruses; Japan; Equine herpesvirus; Epitope; Glycoprotein G; ELISA; Glycoprotein E

A major immunogenic region of equine herpesvirus (EHV)-1 glycoprotein E (gE) was identified. Firstly, the various fragments of EHV-1 gE were expressed as fusion proteins with glutathione S-transferase (GST) in Escherichia coli and their antigenicities were compared by immunoblot analysis using sera...