Electron transfer engineering of artificially designed cell factory for complete biosynthesis of steroids - Chen, Qihang; Wei, Wenqian; Chao, Zikai; Qi, Rui; He, Jianhong; Chen, Huating; Wang, Ke; Wang, Xinglong; Rao, Yijian; Zhou, Jingwen (Nature communications, 2025)
Keywords: 119/118; 140/131; 38/70; 631/326/2522; 631/553/552; 631/61/318; 631/92/552; 7-Dehydrocholesterol reductase; 82/80; Adenine; Biosynthesis; Cholesterol; Cholesterol - biosynthesis; Cholesterol Side-Chain Cleavage Enzyme - genetics; Cholesterol Side-Chain Cleavage Enzyme - metabolism; Electron transfer; Electron Transport; Electrons; Engineering; Enzymes; Factories; Humanities and Social Sciences; Industrial engineering; Metabolic Engineering - methods; multidisciplinary; NADP - metabolism; NADPH-diaphorase; Nicotinamide; Nicotinamide adenine dinucleotide; Oxidoreductases Acting on CH-CH Group Donors - genetics; Oxidoreductases Acting on CH-CH Group Donors - metabolism; Pregnenolone; Pregnenolone - biosynthesis; Reductases; Regeneration; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Science; Science (multidisciplinary); Steroid hormones; Steroids; Steroids - biosynthesis; Synthetic Biology - methods