Bivalent single-domain antibodies show potent mpox virus neutralization through M1R antigen

• Published in: Communications biology Vol. 8; no. 1; pp. 1073 - 11
• Main Authors: Akazawa, Daisuke, Shimojima, Masayuki, Park, Eun-Sil, Okutani, Akiko, Virhuez-Mendoza, Milagros, Inoue, Yusuke, Hishiki, Takayuki, Maeda, Ken, Ebihara, Hideki, Takahashi, Yoshimasa, Watashi, Koichi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18.07.2025; Nature Publishing Group; Nature Portfolio
• Subjects: 13/51; 631/326/596/1746; 631/61/51/2318; 82; 82/1; Animals; Antibodies, Neutralizing - immunology; Antibodies, Viral - immunology; Antigens; Antigens, Viral - immunology; Antiviral agents; Biomedical and Life Sciences; Bispecific antibodies; Cloning; Cowpox; Drug development; E coli; Epidemics; Epitopes; Epitopes - immunology; Humans; Infections; Life Sciences; Mice; Mice, Inbred BALB C; Monoclonal antibodies; Monomers; Mpox; Neutralization Tests; Orthopoxvirus - immunology; Phages; Single-Domain Antibodies - immunology; Single-Domain Antibodies - pharmacology; Smallpox; Surface antigens; Vaccines; Viruses; Liberia

Despite the recent mpox outbreak raising global concerns, no fully validated antiviral treatment exists, highlighting the urgent need for effective therapeutics. Here, by taking advantage of the preparation technology for single-domain (VHH) antibodies, we generated VHHs targeting the six major mpox...