AI-driven high-throughput droplet screening of cell-free gene expression - Zhu, Jiawei; Meng, Yaru; Gao, Wenli; Yang, Shuo; Zhu, Wenjie; Ji, Xiangyang; Zhai, Xuanpei; Liu, Wan-Qiu; Luo, Yuan; Ling, Shengjie; Li, Jian; Liu, Yifan (Nature communications, 2025)
Keywords: 38/35; 49/56; 49/62; 631/553/552; 631/92/507; 639/638/11/877; Bacillus subtilis - genetics; Bacillus subtilis - metabolism; Cell-Free System; Color coding; Combinatorial analysis; Composition; Constraints; Cost effectiveness; Droplets; E coli; Efficiency; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Fluorescence; Gene Expression; Green fluorescent protein; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; High-Throughput Screening Assays - methods; Humanities and Social Sciences; Machine Learning; Microfluidics; Microfluidics - methods; multidisciplinary; Optimization; Proteins; Science; Science (multidisciplinary); Screening; Synthetic Biology - methods; Transfer learning