Intestinal permeability of N-acetylcysteine is driven by gut microbiota-dependent cysteine palmitoylation

• Published in: Nature communications Vol. 16; no. 1; pp. 4623 - 15
• Main Authors: Zhang, Yu-Hang, Dai, Chen-Shu, Wang, Ya-Jie, Wang, Wen-Yu, Qi, Tian-Tian, Xia, Man-Cheng, Zhou, Gan, Cui, Yi-Min
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 19.05.2025; Nature Publishing Group; Nature Portfolio
• Subjects: 14/19; 14/34; 49/40; 49/47; 49/90; 49/91; 59/5; 631/154/152; 631/154/436/1729; 64/86; Acetylcysteine; Acetylcysteine - metabolism; Acetylcysteine - pharmacokinetics; Animals; Bacteroides; Classification; Cysteine; Cysteine - metabolism; Drug interaction; Female; Gastrointestinal Microbiome - physiology; Germ-Free Life; Germfree; Gut microbiota; Humanities and Social Sciences; Humans; Intestinal Barrier Function; Intestinal microflora; Intestinal Mucosa - metabolism; Lipoylation; Machine learning; Male; Metabolomics; Microbiomes; Microbiota; Microorganisms; multidisciplinary; Oral administration; Palmitoylation; Perfusion; Permeability; Prediction models; Rats; Rats, Sprague-Dawley; Science; Science (multidisciplinary); Specific pathogen free; Specific Pathogen-Free Organisms

Trillions of intestinal microbiota are essential to the permeability of orally administered drugs. However, identifying microbial-drug interactions remains challenging due to the highly variable composition of intestinal flora among individuals. Using single-pass intestinal perfusion (SPIP) platform...