Investigating sample pooling strategies for DIGE experiments to address biological variability

• Published in: Proteomics (Weinheim) Vol. 9; no. 2; pp. 388 - 397
• Main Authors: Karp, Natasha A, Lilley, Kathryn S
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 01.01.2009; WILEY‐VCH Verlag; Wiley-VCH
• Subjects: Analysis of Variance; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Biological variation; Brain Chemistry; Carbocyanines - chemistry; DIGE; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel - methods; Fluorescent Dyes - chemistry; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling - methods; Genetic Variation; Humans; Mice; Miscellaneous; Models, Statistical; Oligonucleotide Array Sequence Analysis; Pooling; Power; Proteins; Reproducibility of Results; Statistics, Nonparametric; Two‐dimensional gel electrophoresis; Biological variation; DIGE; Proteomics; Two-dimensional gel electrophoresis; Two dimensional electrophoresis; Pooling; Power

If biological questions are to be answered using quantitative proteomics, it is essential to design experiments which have sufficient power to be able to detect changes in expression. Sample subpooling is a strategy that can be used to reduce the variance but still allow studies to encompass biologi...