Fine‐tuning the amylose content of rice by precise base editing of the Wx gene
The genetic diversity and phenotypic variability of crop agronomic traits is valued by breeders for their benefits in crop breeding but are limited for most target traits. The rice Waxy (Wx) gene (LOC_Os06g04200) encodes granule‐bound starch synthase I (GBSSI), which determines the amylose content (...
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| Published in | Plant biotechnology journal Vol. 19; no. 1; pp. 11 - 13 |
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| Main Authors | , , , , , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
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England
John Wiley & Sons, Inc
01.01.2021
John Wiley and Sons Inc |
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| Abstract | The genetic diversity and phenotypic variability of crop agronomic traits is valued by breeders for their benefits in crop breeding but are limited for most target traits. The rice Waxy (Wx) gene (LOC_Os06g04200) encodes granule‐bound starch synthase I (GBSSI), which determines the amylose content (AC) of endosperm by controlling amylose synthesis. The number of altered bases in each line (coloured in red) is indicated by the letter S followed by a number. (c) A structural model of Wxb constructed using the PROTEIN DATA BANK server; mutated residues contributing to the changes of AC are shown as spheres and are coloured (P124 in apricot, R125 in blue, R158 in red violet, G159 in white, V160 in green, D161 in red, T178 in orange and Y191 in purple). (d) Analysis of potential off‐target sites in the seven T1 edited lines. To determine the effect of these mutations on AC, we measured the apparent amylose contents (AACs) of grains from the seven mutant lines (Wxm5‐Wxm11), NIP (Wxb) and a 'soft rice' control Nangeng9108 (NG9108) (Wxmp) (Figure 1e). |
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| AbstractList | The genetic diversity and phenotypic variability of crop agronomic traits is valued by breeders for their benefits in crop breeding but are limited for most target traits. The rice Waxy (Wx) gene (LOC_Os06g04200) encodes granule‐bound starch synthase I (GBSSI), which determines the amylose content (AC) of endosperm by controlling amylose synthesis. The number of altered bases in each line (coloured in red) is indicated by the letter S followed by a number. (c) A structural model of Wxb constructed using the PROTEIN DATA BANK server; mutated residues contributing to the changes of AC are shown as spheres and are coloured (P124 in apricot, R125 in blue, R158 in red violet, G159 in white, V160 in green, D161 in red, T178 in orange and Y191 in purple). (d) Analysis of potential off‐target sites in the seven T1 edited lines. To determine the effect of these mutations on AC, we measured the apparent amylose contents (AACs) of grains from the seven mutant lines (Wxm5‐Wxm11), NIP (Wxb) and a 'soft rice' control Nangeng9108 (NG9108) (Wxmp) (Figure 1e). |
| Author | Chen, Zhihui Zhu, Qian‐Hao Fan, Fangjun Bu, Qingyun Lin, Qiupeng Gao, Caixia Wang, Jun Wei, Xiaodong Tao, Yajun Jiang, Yanjie Xu, Yang Wang, Fangquan Li, Xiufeng Li, Wenqi Yang, Jie Zhang, Rui |
| AuthorAffiliation | 3 State Key Laboratory of Plant Cell and Chromosome Engineering Center for Genome Editing Institute of Genetics and Developmental Biology Innovation Academy for Seed Design Chinese Academy of Sciences Beijing China 4 College of Advanced Agricultural Sciences University of Chinese Academy of Sciences Beijing China 1 Institute of Food Crops Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice Improvement Nanjing China 6 CSIRO Agriculture and Food Canberra ACT Australia 2 Jiangsu Co‐Innovation Center for Modern Production Technology of Grain Crops Yangzhou University Yangzhou China 5 Northeast Institute of Geography and Agroecology Key Laboratory of Soybean Molecular Design Breeding Chinese Academy of Sciences Harbin China |
| AuthorAffiliation_xml | – name: 3 State Key Laboratory of Plant Cell and Chromosome Engineering Center for Genome Editing Institute of Genetics and Developmental Biology Innovation Academy for Seed Design Chinese Academy of Sciences Beijing China – name: 2 Jiangsu Co‐Innovation Center for Modern Production Technology of Grain Crops Yangzhou University Yangzhou China – name: 1 Institute of Food Crops Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice Improvement Nanjing China – name: 5 Northeast Institute of Geography and Agroecology Key Laboratory of Soybean Molecular Design Breeding Chinese Academy of Sciences Harbin China – name: 6 CSIRO Agriculture and Food Canberra ACT Australia – name: 4 College of Advanced Agricultural Sciences University of Chinese Academy of Sciences Beijing China |
| Author_xml | – sequence: 1 givenname: Yang surname: Xu fullname: Xu, Yang organization: Yangzhou University – sequence: 2 givenname: Qiupeng surname: Lin fullname: Lin, Qiupeng organization: University of Chinese Academy of Sciences – sequence: 3 givenname: Xiufeng surname: Li fullname: Li, Xiufeng organization: Chinese Academy of Sciences – sequence: 4 givenname: Fangquan surname: Wang fullname: Wang, Fangquan organization: Yangzhou University – sequence: 5 givenname: Zhihui surname: Chen fullname: Chen, Zhihui organization: Yangzhou University – sequence: 6 givenname: Jun surname: Wang fullname: Wang, Jun organization: Yangzhou University – sequence: 7 givenname: Wenqi surname: Li fullname: Li, Wenqi organization: Yangzhou University – sequence: 8 givenname: Fangjun surname: Fan fullname: Fan, Fangjun organization: Yangzhou University – sequence: 9 givenname: Yajun surname: Tao fullname: Tao, Yajun organization: Yangzhou University – sequence: 10 givenname: Yanjie surname: Jiang fullname: Jiang, Yanjie organization: Yangzhou University – sequence: 11 givenname: Xiaodong surname: Wei fullname: Wei, Xiaodong organization: Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice Improvement – sequence: 12 givenname: Rui surname: Zhang fullname: Zhang, Rui organization: Chinese Academy of Sciences – sequence: 13 givenname: Qian‐Hao surname: Zhu fullname: Zhu, Qian‐Hao organization: CSIRO Agriculture and Food – sequence: 14 givenname: Qingyun orcidid: 0000-0002-5386-3577 surname: Bu fullname: Bu, Qingyun email: buqingyun@iga.ac.cn organization: Chinese Academy of Sciences – sequence: 15 givenname: Jie orcidid: 0000-0003-2355-3022 surname: Yang fullname: Yang, Jie email: yangjie168@aliyun.com organization: Yangzhou University – sequence: 16 givenname: Caixia orcidid: 0000-0003-3169-8248 surname: Gao fullname: Gao, Caixia email: cxgao@genetics.ac.cn organization: University of Chinese Academy of Sciences |
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| Cites_doi | 10.1016/j.carbpol.2018.04.065 10.1038/nbt.3811 10.1016/j.molp.2015.04.007 10.1111/jipb.12620 10.1016/j.foodchem.2015.09.112 10.1021/acs.jafc.8b02913 10.1271/bbb.120305 10.1126/science.aax0025 10.1007/s11032-011-9647-x |
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| Copyright | 2020 The Authors. published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
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| Keywords | Wx rice base editing eating and cooking quality amylose content appearance quality |
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| References | 2019; 366 2018; 195 2018; 60 2015; 29 2018; 66 2015; 8 2012; 76 2016; 196 2012; 30 2017; 35 Zhu J. (e_1_2_5_10_1) 2015; 29 e_1_2_5_9_1 e_1_2_5_8_1 e_1_2_5_11_1 e_1_2_5_7_1 e_1_2_5_6_1 e_1_2_5_5_1 e_1_2_5_4_1 e_1_2_5_3_1 e_1_2_5_2_1 |
| References_xml | – volume: 66 start-page: 9750 year: 2018 end-page: 9760 article-title: Down‐regulation of gene expression results in novel low‐amylose rice with soft, transparent grains publication-title: J. Agric. Food Chem. – volume: 29 start-page: 431 year: 2015 end-page: 438 article-title: Progress in the allelic variation of gene and its application in rice breeding publication-title: Chinese J. Rice Sci. – volume: 76 start-page: 1591 year: 2012 end-page: 1595 article-title: Interdomain disulfide bridge in the rice granule bound starch synthase I catalytic domain as elucidated by X‐Ray structure analysis publication-title: Biosci. Biotech. Bioch. – volume: 196 start-page: 702 year: 2016 end-page: 711 article-title: The importance of amylose and amylopectin fine structure for textural properties of cooked rice grains publication-title: Food Chem. – volume: 35 start-page: 438 year: 2017 end-page: 440 article-title: Precise base editing in rice, wheat and maize with a Cas9‐cytidine deaminase fusion publication-title: Nat. Biotechnol. – volume: 30 start-page: 583 year: 2012 end-page: 595 article-title: Detection of allelic variation at the locus with single‐segment substitution lines in rice ( L.) publication-title: Mol. Breed. – volume: 195 start-page: 9 year: 2018 end-page: 17 article-title: Starch molecular structure: The basis for an improved understanding of cooked rice texture publication-title: Carbohyd. Polym. – volume: 8 start-page: 1274 year: 2015 end-page: 1284 article-title: A robust CRISPR/Cas9 system for convenient, high‐efficiency multiplex genome editing in monocot and dicot plants publication-title: Mol. Plant. – volume: 366 start-page: x25 year: 2019 article-title: Revolutions in agriculture chart a course for targeted breeding of old and new crops publication-title: Science – volume: 60 start-page: 369 year: 2018 end-page: 375 article-title: Generation of new glutinous rice by CRISPR/Cas9‐targeted mutagenesis of the gene in elite rice varieties publication-title: J. Integr. Plant Biol. – ident: e_1_2_5_3_1 doi: 10.1016/j.carbpol.2018.04.065 – ident: e_1_2_5_11_1 doi: 10.1038/nbt.3811 – ident: e_1_2_5_6_1 doi: 10.1016/j.molp.2015.04.007 – ident: e_1_2_5_9_1 doi: 10.1111/jipb.12620 – ident: e_1_2_5_4_1 doi: 10.1016/j.foodchem.2015.09.112 – ident: e_1_2_5_5_1 doi: 10.1021/acs.jafc.8b02913 – ident: e_1_2_5_7_1 doi: 10.1271/bbb.120305 – ident: e_1_2_5_2_1 doi: 10.1126/science.aax0025 – ident: e_1_2_5_8_1 doi: 10.1007/s11032-011-9647-x – volume: 29 start-page: 431 year: 2015 ident: e_1_2_5_10_1 article-title: Progress in the allelic variation of Wx gene and its application in rice breeding publication-title: Chinese J. Rice Sci. |
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| SubjectTerms | Agronomic crops Agronomy Amino acids Amylose amylose content appearance quality base editing Brief Communication Brief Communications eating and cooking quality Endosperm Genetic diversity Genetic variability Granule-bound starch synthase Mutation Oryza - genetics Plant breeding Plant Proteins - genetics Proteins Rice Seeds Starch Synthase Structural models Waxy gene |
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| Title | Fine‐tuning the amylose content of rice by precise base editing of the Wx gene |
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