gsdf is a downstream gene of dmrt1 that functions in the male sex determination pathway of the Nile tilapia

• Published in: Molecular reproduction and development Vol. 83; no. 6; pp. 497 - 508
• Main Authors: Jiang, Dong-Neng, Yang, Hui-Hui, Li, Ming-Hui, Shi, Hong-Juan, Zhang, Xian-Bo, Wang, De-Shou
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.06.2016; Wiley Subscription Services, Inc
• Subjects: Animals; Fish Proteins - genetics; Fish Proteins - metabolism; Male; Oreochromis niloticus; Sex Determination Processes - physiology; Teleostei; Testis - embryology; Tilapia; Tilapia - embryology; Tilapia - genetics; Transcription Factors - genetics; Transcription Factors - metabolism; Transforming Growth Factor beta - genetics; Transforming Growth Factor beta - metabolism
• ISSN: 1040-452X; 1098-2795; 1098-2795
• DOI: 10.1002/mrd.22642

SUMMARY Gonadal soma‐derived factor (gsdf) is critical for testicular differentiation in teleosts, yet detailed analysis of Gsdf on testicular differentiation is lacking. In the present study, we knocked out tilapia gsdf using CRISPR/Cas9. F0 gsdf‐deficient XY fish with high mutation rate (≥58%) dev...