Fatty-Acid Uptake in Prostate Cancer Cells Using Dynamic Microfluidic Raman Technology

It is known that intake of dietary fatty acid (FA) is strongly correlated with prostate cancer progression but is highly dependent on the type of FAs. High levels of palmitic acid (PA) or arachidonic acid (AA) can stimulate the progression of cancer. In this study, a unique experimental set-up consi...

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Bibliographic Details
Published inMolecules (Basel, Switzerland) Vol. 25; no. 7; p. 1652
Main Authors Tang, Nga-Tsing, D Snook, Richard, Brown, Mick D, Haines, Bryan A, Ridley, Andrew, Gardner, Peter, Denbigh, Joanna L
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 03.04.2020
MDPI
Subjects
Arachidonic acid
Arachidonic Acid - analysis
Competition
Deuteration
Dietary intake
Docosahexaenoic acid
Docosahexaenoic Acids - pharmacology
Eicosapentaenoic acid
Eicosapentaenoic Acid - pharmacology
Experiments
Fatty acids
Fatty Acids, Omega-3 - pharmacology
Flow cytometry
Humans
Lipids
Male
Mass spectrometry
Metabolism
microfluidic device
Microfluidics
Palmitic acid
Palmitic Acid - analysis
PC-3 Cells
Prostate cancer
Prostatic Neoplasms - chemistry
Raman spectroscopy
Scientific imaging
Shear flow
Single-Cell Analysis
Spectrum Analysis, Raman
Studies
prostate cancer
microfluidic device
fatty acids
Raman spectroscopy
single cell analysis
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Summary:It is known that intake of dietary fatty acid (FA) is strongly correlated with prostate cancer progression but is highly dependent on the type of FAs. High levels of palmitic acid (PA) or arachidonic acid (AA) can stimulate the progression of cancer. In this study, a unique experimental set-up consisting of a Raman microscope, coupled with a commercial shear-flow microfluidic system is used to monitor fatty acid uptake by prostate cancer (PC-3) cells in real-time at the single cell level. Uptake of deuterated PA, deuterated AA, and the omega-3 fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were monitored using this new system, while complementary flow cytometry experiments using Nile red staining, were also conducted for the validation of the cellular lipid uptake. Using this novel experimental system, we show that DHA and EPA have inhibitory effects on the uptake of PA and AA by PC-3 cells.
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ISSN:1420-3049
1420-3049
DOI:10.3390/molecules25071652