Dual Orientation of the Outer Membrane Lipoprotein P6 of Nontypeable Haemophilus influenzae

• Published in: Journal of Bacteriology Vol. 195; no. 14; pp. 3252 - 3259
• Main Authors: Michel, Lea Vacca, Snyder, Joy, Schmidt, Rachel, Milillo, Jennifer, Grimaldi, Kyle, Kalmeta, Breanna, Khan, M. Nadeem, Sharma, Sharad, Wright, Leslie Kate, Pichichero, Michael E
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.07.2013
• Subjects: antibacterial properties; Antibodies, Bacterial - metabolism; Antibodies, Monoclonal - metabolism; Bacterial Outer Membrane Proteins - analysis; Bacteriology; blood serum; Blotting, Western; Cell Membrane - chemistry; Cells; E coli; epitopes; Escherichia coli; Flow Cytometry; Gram-negative bacteria; Haemophilus influenzae; Haemophilus influenzae - chemistry; Haemophilus Vaccines - analysis; humans; image analysis; Lipoproteins; Lipoproteins - analysis; Membranes; microscopy; Microscopy, Confocal; Monoclonal antibodies; mutants; Proteases; proteinases; Staining and Labeling; vaccines; Western blotting

The majority of outer membrane (OM) lipoproteins in Gram-negative bacteria are tethered to the membrane via an attached lipid moiety and oriented facing in toward the periplasmic space; a few lipoproteins have been shown to be surface exposed. The outer membrane lipoprotein P6 from the Gram-negative...