Development of an LC⁻Tandem Mass Spectrometry Method for the Quantitative Analysis of Hercynine in Human Whole Blood

• Published in: Molecules (Basel, Switzerland) Vol. 23; no. 12; p. 3326
• Main Authors: Sotgia, Salvatore, Murphy, Rhys B, Zinellu, Angelo, Elliot, David, Paliogiannis, Panagiotis, Pinna, Gerard Aimè, Carru, Ciriaco, Mangoni, Arduino A
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 14.12.2018; MDPI
• Subjects: Acetonitrile; Acids; aminothione; antioxidant; Biological activity; Biosynthesis; Blood levels; Calibration; Cold water; Crosstalk; Deuteration; dismutatiion; Erythrocytes; Formic acid; LC-tandem mass spectrometry; Lysis; Mass spectrometry; Mass spectroscopy; Metabolites; Oxidative stress; Physiology; Quantitative analysis; Retention; Scientific imaging; Water purification; zwitterion; dismutatiion; antioxidant; zwitterion; LC-tandem mass spectrometry; aminothione

Given that the peculiar redox behavior of ergothioneine involves a rapid regeneration process, the measurement of its precursor and redox metabolite hercynine could be particularly useful in assessing its role in oxidative stress or other biological processes. Thus, a LC-MS/MS method for the determi...