A Subcellular Quantitative Proteomic Analysis of Herpes Simplex Virus Type 1-Infected HEK 293T Cells

• Published in: Molecules (Basel, Switzerland) Vol. 24; no. 23; p. 4215
• Main Authors: Wan, Weiwei, Wang, Liangjie, Chen, Xi, Zhu, Shenglin, Shang, Weijuan, Xiao, Gengfu, Zhang, Lei-Ke
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20.11.2019; MDPI
• Subjects: Bioinformatics; chchd2; Cytoplasm; Deoxyribonucleic acid; DNA; DNA biosynthesis; DNA Replication - physiology; DNA viruses; DNA, Viral - biosynthesis; DNA, Viral - genetics; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Fractionation; Gene expression; Glycoproteins; HEK293 Cells; Herpes simplex; herpes simplex virus type 1; Herpes viruses; Herpesvirus 1, Human - physiology; Humans; ifitm3; Infections; Interferon; Kinases; Latency; Mass spectrometry; Mass spectroscopy; Membrane Proteins - genetics; Membrane Proteins - metabolism; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Peptides; Proteins; Proteomics; quantitative proteomics; Regulation; RNA polymerase; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Signal transduction; Stimulators; Toll-like receptors; Transcription Factors - genetics; Transcription Factors - metabolism; trim27; Viral infections; Virus Replication - physiology; Viruses; virus–host interaction; IFITM3; herpes simplex virus type 1; CHCHD2; virus–host interaction; Quantitative proteomics; TRIM27
• DOI: 10.3390/molecules24234215
• ISSN: 1420-3049

Herpes simplex virus type 1 (HSV-1) is widespread double-stranded DNA (dsDNA) virus that establishes life-long latency and causes diverse severe symptoms. The mechanisms of HSV-1 infection and HSV-1's interactions with various host cells have been studied and reviewed extensively. Type I interferons were secreted by host cells upon HSV infection and play a vital role in controlling virus proliferation. A few studies, however, have focused on HSV-1 infection without the presence of interferon (IFN) signaling. In this study, HEK 293T cells with low toll-like receptor (TLR) and stimulator of interferon genes protein (STING) expression were infected with HSV-1 and subjected to a quantitative proteomic analysis. By using a subcellular fractionation strategy and high-performance mass spectrometry, a total of 6607 host proteins were quantified, of which 498 proteins were differentially regulated. A bioinformatics analysis indicated that multiple signaling pathways might be involved in HSV-1 infection. A further functional study indicated the role of Interferon-induced transmembrane protein 3 (IFITM3), Coiled-coil-helix-coiled-coil-helix domain-containing protein 2 (CHCHD2), and Tripartite motif-containing protein 27 (TRIM27) in inhibiting viral DNA replication and proliferation. Our data provide a global view of host responses to HSV-1 infection in HEK 293T cells and identify the proteins involved in the HSV-1 infection process.