MIRU-VNTR typing adds discriminatory value to groups of Mycobacterium bovis and Mycobacterium caprae strains defined by spoligotyping
The value of Mycobacterial Interspersed Repetitive Units-Variable Number Tandem Repeats (MIRU-VNTR) as a genotyping technique for Mycobacterium bovis and Mycobacterium caprae, has been confirmed in different countries and epidemiological scenarios. However, a standardized panel of loci has not yet b...
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Published in | Veterinary microbiology Vol. 143; no. 2; pp. 299 - 306 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier B.V
14.07.2010
Amsterdam; New York: Elsevier Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | The value of Mycobacterial Interspersed Repetitive Units-Variable Number Tandem Repeats (MIRU-VNTR) as a genotyping technique for
Mycobacterium bovis and
Mycobacterium caprae, has been confirmed in different countries and epidemiological scenarios. However, a standardized panel of
loci has not yet been adopted for these species, since allelic diversity of each
locus differs among countries. To determine the most discriminatory
loci, a panel of 181
M. bovis and
M. caprae strains representing 12 spoligotypes was created. The panel included strains from the three predominant spoligotypes previously isolated in Portugal; strains from spoligotyping group SB0140, prevalent in the British Isles but also detected in Portugal; strains from spoligotypes common to cattle and wildlife species and strains from the
M. caprae spoligotyping group SB0157. MIRU-VNTR analysis of these strains, targeting 8 selected
loci, produced 87 different profiles (
h
=
0.99), being VNTR3232, QUB11a, ETR-B and ETR-A the most discriminatory
loci (
h
=
0.96). A single
M. bovis spoligotyping group could be differentiated – up to 44 MIRU-VNTR profiles. These results emphasize the high genotype diversity of Portuguese isolates compared with other countries. MIRU-VNTR typing was superior to spoligotyping for identifying multi-genotype infected herds and the combination of the two genotyping methods by a hierarchical approach confirmed the genetic relatedness of
M. bovis isolates between cattle and wildlife. |
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Bibliography: | http://dx.doi.org/10.1016/j.vetmic.2009.11.027 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 0378-1135 1873-2542 |
DOI: | 10.1016/j.vetmic.2009.11.027 |