Quantification and Validation of an HPLC Method for Low Concentrations of Apigenin-7- O -Glucuronide in Agrimonia pilosa Aqueous Ethanol Extract Topical Cream by Liquid-Liquid Extraction
In this study, we aimed to develop and validate a pretreatment method for separating and analyzing the small amounts of biomarkers contained in topical cream formulations. Analyzing semisolid formulations that contain low concentrations of active ingredients is difficult. Cream formulations containi...
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Published in | Molecules (Basel, Switzerland) Vol. 28; no. 2; p. 713 |
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Abstract | In this study, we aimed to develop and validate a pretreatment method for separating and analyzing the small amounts of biomarkers contained in topical cream formulations. Analyzing semisolid formulations that contain low concentrations of active ingredients is difficult. Cream formulations containing an aqueous ethanol extract of 0.1% Agrimonia pilosa is an example. Approximately 0.0013% of apigenin-7-
-glucuronide(A7OG) was contained as a biomarker in the cream. To determine the A7OG content present in the cream formulation, liquid-liquid extraction using dichlormethane was applied. In addition, the volume of the distribution liquid was measured using the peak ratios of the indicator component, A7OG, and an internal standard, baicalin. Subsequently, the A7OG content in the cream formulation was calculated. Using this time-saving method, A7OG can be simply analyzed without additional pretreatment steps, such as evaporation and reconstitution. Moreover, the validation results confirmed that this analytical method met all of the criteria. Consequently, A7OG was successfully isolated from the cream, analyzed, and quantified using the developed method. |
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AbstractList | In this study, we aimed to develop and validate a pretreatment method for separating and analyzing the small amounts of biomarkers contained in topical cream formulations. Analyzing semisolid formulations that contain low concentrations of active ingredients is difficult. Cream formulations containing an aqueous ethanol extract of 0.1% Agrimonia pilosa is an example. Approximately 0.0013% of apigenin-7-O-glucuronide(A7OG) was contained as a biomarker in the cream. To determine the A7OG content present in the cream formulation, liquid–liquid extraction using dichlormethane was applied. In addition, the volume of the distribution liquid was measured using the peak ratios of the indicator component, A7OG, and an internal standard, baicalin. Subsequently, the A7OG content in the cream formulation was calculated. Using this time-saving method, A7OG can be simply analyzed without additional pretreatment steps, such as evaporation and reconstitution. Moreover, the validation results confirmed that this analytical method met all of the criteria. Consequently, A7OG was successfully isolated from the cream, analyzed, and quantified using the developed method. In this study, we aimed to develop and validate a pretreatment method for separating and analyzing the small amounts of biomarkers contained in topical cream formulations. Analyzing semisolid formulations that contain low concentrations of active ingredients is difficult. Cream formulations containing an aqueous ethanol extract of 0.1% Agrimonia pilosa is an example. Approximately 0.0013% of apigenin-7- -glucuronide(A7OG) was contained as a biomarker in the cream. To determine the A7OG content present in the cream formulation, liquid-liquid extraction using dichlormethane was applied. In addition, the volume of the distribution liquid was measured using the peak ratios of the indicator component, A7OG, and an internal standard, baicalin. Subsequently, the A7OG content in the cream formulation was calculated. Using this time-saving method, A7OG can be simply analyzed without additional pretreatment steps, such as evaporation and reconstitution. Moreover, the validation results confirmed that this analytical method met all of the criteria. Consequently, A7OG was successfully isolated from the cream, analyzed, and quantified using the developed method. In this study, we aimed to develop and validate a pretreatment method for separating and analyzing the small amounts of biomarkers contained in topical cream formulations. Analyzing semisolid formulations that contain low concentrations of active ingredients is difficult. Cream formulations containing an aqueous ethanol extract of 0.1% Agrimonia pilosa is an example. Approximately 0.0013% of apigenin-7- O -glucuronide(A7OG) was contained as a biomarker in the cream. To determine the A7OG content present in the cream formulation, liquid–liquid extraction using dichlormethane was applied. In addition, the volume of the distribution liquid was measured using the peak ratios of the indicator component, A7OG, and an internal standard, baicalin. Subsequently, the A7OG content in the cream formulation was calculated. Using this time-saving method, A7OG can be simply analyzed without additional pretreatment steps, such as evaporation and reconstitution. Moreover, the validation results confirmed that this analytical method met all of the criteria. Consequently, A7OG was successfully isolated from the cream, analyzed, and quantified using the developed method. |
Author | Kim, Woo Kyung Kim, Hyun Jong Nam, Yu Ran Lee, Jin Seok |
AuthorAffiliation | 1 Department of Internal Medicine, Graduate School of Medicine, Dongguk University, 27 Dongguk-ro, Goyang 10326, Republic of Korea 2 Department of Physiology, College of Medicine, Dongguk University, 123 Dongdae-ro, Gyeongju 38066, Republic of Korea 3 Channelopathy Research Center (CRC), College of Medicine, Dongguk University, 32 Dongguk-ro, Goyang 10326, Republic of Korea |
AuthorAffiliation_xml | – name: 3 Channelopathy Research Center (CRC), College of Medicine, Dongguk University, 32 Dongguk-ro, Goyang 10326, Republic of Korea – name: 1 Department of Internal Medicine, Graduate School of Medicine, Dongguk University, 27 Dongguk-ro, Goyang 10326, Republic of Korea – name: 2 Department of Physiology, College of Medicine, Dongguk University, 123 Dongdae-ro, Gyeongju 38066, Republic of Korea |
Author_xml | – sequence: 1 givenname: Jin Seok orcidid: 0000-0002-1909-8955 surname: Lee fullname: Lee, Jin Seok organization: Department of Internal Medicine, Graduate School of Medicine, Dongguk University, 27 Dongguk-ro, Goyang 10326, Republic of Korea – sequence: 2 givenname: Yu Ran orcidid: 0000-0001-7816-2585 surname: Nam fullname: Nam, Yu Ran organization: Channelopathy Research Center (CRC), College of Medicine, Dongguk University, 32 Dongguk-ro, Goyang 10326, Republic of Korea – sequence: 3 givenname: Hyun Jong orcidid: 0000-0002-6130-7028 surname: Kim fullname: Kim, Hyun Jong organization: Channelopathy Research Center (CRC), College of Medicine, Dongguk University, 32 Dongguk-ro, Goyang 10326, Republic of Korea – sequence: 4 givenname: Woo Kyung orcidid: 0000-0003-0359-3967 surname: Kim fullname: Kim, Woo Kyung organization: Channelopathy Research Center (CRC), College of Medicine, Dongguk University, 32 Dongguk-ro, Goyang 10326, Republic of Korea |
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Cites_doi | 10.11002/kjfp.2015.22.2.261 10.3390/pr9091631 10.3390/cimb44080229 10.52361/fsbh.2021.1.e10 10.5740/jaoacint.18-0378 10.1016/j.fuel.2018.12.130 10.21037/atm.2017.03.17 10.2174/2213240601666141113212732 10.3892/etm.2016.3305 10.1055/s-0028-1088393 10.1055/s-0043-118534 10.2174/1874402800902010012 10.1155/2022/3742208 10.1186/s43094-020-00091-5 10.1016/B978-0-12-800871-3.00003-1 10.1016/j.heliyon.2022.e09972 10.1021/ed084p1982 |
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SubjectTerms | Agrimonia Agrimonia pilosa apigenin-7-O-glucuronide Biomarkers Chromatography, High Pressure Liquid cream Ethanol Formulations HPLC-DAD Liquid-Liquid Extraction Plant Extracts Quality control Solvents validation Water |
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Title | Quantification and Validation of an HPLC Method for Low Concentrations of Apigenin-7- O -Glucuronide in Agrimonia pilosa Aqueous Ethanol Extract Topical Cream by Liquid-Liquid Extraction |
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