Determination of Ascorbic Acid, Total Ascorbic Acid, and Dehydroascorbic Acid in Bee Pollen Using Hydrophilic Interaction Liquid Chromatography-Ultraviolet Detection

• Published in: Molecules (Basel, Switzerland) Vol. 25; no. 23; p. 5696
• Main Authors: Zhu, Meifei, Tang, Jian, Tu, Xijuan, Chen, Wenbin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 03.12.2020; MDPI
• Subjects: Acids; Animals; ascorbic acid; Ascorbic Acid - chemistry; bee pollen; Bees; Bees - chemistry; Brassica - chemistry; Camellia - chemistry; Chromatography; Chromatography, High Pressure Liquid - methods; dehydroascorbic acid; Dehydroascorbic Acid - chemistry; Food; HILIC; Hydrophobic and Hydrophilic Interactions; Lotus - chemistry; Phosphorous Acids - chemistry; Pollen - chemistry; total ascorbic acids; Ultraviolet Rays; dehydroascorbic acid; ascorbic acid; total ascorbic acids; HILIC; bee pollen

Ascorbic acid (AA) is one of the essential nutrients in bee pollen, however, it is unstable and likely to be oxidized. Generally, the oxidation form (dehydroascorbic acid (DHA)) is considered to have equivalent biological activity as the reduction form. Thus, determination of the total content of AA...