Exploitation of Vitis vinifera , Foeniculum vulgare , Cannabis sativa and Punica granatum By-Product Seeds as Dermo-Cosmetic Agents

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 3; p. 731
• Main Authors: Michailidis, Dimitris, Angelis, Apostolis, Nikolaou, Panagiota Efstathia, Mitakou, Sofia, Skaltsounis, Alexios Léandros
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 31.01.2021; MDPI
• Subjects: Aging; Aging - drug effects; Antioxidants; Byproducts; Cannabis; Cannabis - chemistry; Cannabis sativa; Carbon dioxide; Chromatography; Cold; Collagen; Collagenase; Cosmetics; Cosmetics - pharmacology; Elastase; Enzyme Inhibitors - pharmacology; Ethanol; fennel; Flowers & plants; Foeniculum - chemistry; Foeniculum vulgare; Food; grapevine; Grapevines; Hemp; Herbal medicine; Hexanes; High performance liquid chromatography; Liquid chromatography; Marijuana; Medicinal plants; Pastes; Plant extracts; Plant Extracts - pharmacology; pomegranate; Pomegranate - chemistry; Proanthocyanidins; Punica granatum; R&D; Raw materials; Research & development; seed by-products; Seeds; Seeds - chemistry; Skin; Skin - drug effects; Solvents; supercritical fluid extraction; Supercritical fluids; Supercritical gas extraction; Tyrosinase; Ultrasound; Vitis - chemistry; Vitis vinifera; anti-collagenase; hemp; supercritical fluid extraction; grapevine; ultrasound extraction; fennel; pomegranate; anti-tyrosinase; anti-elastase; seed by-products
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26030731

In the current study, by-product seed pastes (VSPs) from , , and , generated during the oil production process, were investigated for their potential exploitation as dermo-cosmetic agent. The extraction pipeline of all the raw materials was developed with emphasis on green methodologies and employed on laboratory scale based on industry-adopted techniques. Two different protocols were applied, Supercritical Fluid Extraction (SFE) and Ultrasound Assisted Extraction (UAE); the by-product pastes were defatted with supercritical CO and Hexane, respectively. Then, two SFE extracts (CO with 10% and 20% of ethanol as co-solvent) and two UAE extracts (with ethanol and ethanol/water 1:1 / ) were obtained from each raw material. The providing yield range was between 2.6 to 76.3 mg/g raw material. The extracts were analyzed with High-Performance Liquid Chromatography coupled with Diode Array Detector (HPLC-DAD) and Liquid Chromatography coupled with High-Resolution Mass Spectrometer (LC-HRMS), and the major compounds, were identified. All the extracts were evaluated for their antioxidant and inhibition activity against collagenase, elastase and tyrosinase enzymes. Grapevine by-product extracts found rich in proanthocyanidins and presented the higher inhibition activity. A holistic green experimental methodology is proposed for the obtainment of extracts from significant medicinal plants by-products that provides us with promising results concerning dermo-cosmetic properties, especially for grape seeds extracts.