Electrophysiological Properties of Human Cortical Organoids: Current State of the Art and Future Directions
Human cortical development is an intricate process resulting in the generation of many interacting cell types and long-range connections to and from other brain regions. Human stem cell-derived cortical organoids are now becoming widely used to model human cortical development both in physiological...
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| Published in | Frontiers in molecular neuroscience Vol. 15; p. 839366 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
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16.02.2022
Frontiers Media S.A |
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| Abstract | Human cortical development is an intricate process resulting in the generation of many interacting cell types and long-range connections to and from other brain regions. Human stem cell-derived cortical organoids are now becoming widely used to model human cortical development both in physiological and pathological conditions, as they offer the advantage of recapitulating human-specific aspects of corticogenesis that were previously inaccessible. Understanding the electrophysiological properties and functional maturation of neurons derived from human cortical organoids is key to ensure their physiological and pathological relevance. Here we review existing data on the electrophysiological properties of neurons in human cortical organoids, as well as recent advances in the complexity of cortical organoid modeling that have led to improvements in functional maturation at single neuron and neuronal network levels. Eventually, a more comprehensive and standardized electrophysiological characterization of these models will allow to better understand human neurophysiology, model diseases and test novel treatments. |
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| AbstractList | Human cortical development is an intricate process resulting in the generation of many interacting cell types and long-range connections to and from other brain regions. Human stem cell-derived cortical organoids are now becoming widely used to model human cortical development both in physiological and pathological conditions, as they offer the advantage of recapitulating human-specific aspects of corticogenesis that were previously inaccessible. Understanding the electrophysiological properties and functional maturation of neurons derived from human cortical organoids is key to ensure their physiological and pathological relevance. Here we review existing data on the electrophysiological properties of neurons in human cortical organoids, as well as recent advances in the complexity of cortical organoid modeling that have led to improvements in functional maturation at single neuron and neuronal network levels. Eventually, a more comprehensive and standardized electrophysiological characterization of these models will allow to better understand human neurophysiology, model diseases and test novel treatments. Human cortical development is an intricate process resulting in the generation of many interacting cell types and long-range connections to and from other brain regions. Human stem cell-derived cortical organoids are now becoming widely used to model human cortical development both in physiological and pathological conditions, as they offer the advantage of recapitulating human-specific aspects of corticogenesis that were previously inaccessible. Understanding the electrophysiological properties and functional maturation of neurons derived from human cortical organoids is key to ensure their physiological and pathological relevance. Here we review existing data on the electrophysiological properties of neurons in human cortical organoids, as well as recent advances in the complexity of cortical organoid modelling that have led to improvements in functional maturation at single neuron and neuronal network levels. Eventually, a more comprehensive and standardized electrophysiological characterization of these models will allow to better understand human neurophysiology, model diseases and test novel treatments. |
| Author | Zourray, Clara Kurian, Manju A Lignani, Gabriele Barral, Serena |
| AuthorAffiliation | 3 Department of Pharmacology, UCL School of Pharmacy , London , United Kingdom 1 Department of Clinical and Experimental Epilepsy, UCL Queen Square Institute of Neurology , London , United Kingdom 4 Department of Neurology, Great Ormond Street Hospital for Children , London , United Kingdom 2 Developmental Neurosciences, Zayed Centre for Research Into Rare Disease in Children, GOS-Institute of Child Health, University College London , London , United Kingdom |
| AuthorAffiliation_xml | – name: 1 Department of Clinical and Experimental Epilepsy, UCL Queen Square Institute of Neurology , London , United Kingdom – name: 4 Department of Neurology, Great Ormond Street Hospital for Children , London , United Kingdom – name: 3 Department of Pharmacology, UCL School of Pharmacy , London , United Kingdom – name: 2 Developmental Neurosciences, Zayed Centre for Research Into Rare Disease in Children, GOS-Institute of Child Health, University College London , London , United Kingdom |
| Author_xml | – sequence: 1 givenname: Clara surname: Zourray fullname: Zourray, Clara organization: Department of Pharmacology, UCL School of Pharmacy, London, United Kingdom – sequence: 2 givenname: Manju A surname: Kurian fullname: Kurian, Manju A organization: Department of Neurology, Great Ormond Street Hospital for Children, London, United Kingdom – sequence: 3 givenname: Serena surname: Barral fullname: Barral, Serena organization: Developmental Neurosciences, Zayed Centre for Research Into Rare Disease in Children, GOS-Institute of Child Health, University College London, London, United Kingdom – sequence: 4 givenname: Gabriele surname: Lignani fullname: Lignani, Gabriele organization: Department of Clinical and Experimental Epilepsy, UCL Queen Square Institute of Neurology, London, United Kingdom |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/35250479$$D View this record in MEDLINE/PubMed |
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| Copyright | Copyright © 2022 Zourray, Kurian, Barral and Lignani. 2022. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. Copyright © 2022 Zourray, Kurian, Barral and Lignani. 2022 Zourray, Kurian, Barral and Lignani |
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| Keywords | network activity neuronal maturation electrophysiology neurodevelopmental disorders cortical organoids |
| Language | English |
| License | Copyright © 2022 Zourray, Kurian, Barral and Lignani. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
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| Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 Reviewed by: Ashwin S. Shetty, Harvard University, United States; Jinsoo Seo, Daegu Gyeongbuk Institute of Science and Technology (DGIST), South Korea These authors share last authorship Edited by: Hyunsoo Shawn Je, Duke-NUS Medical School, Singapore This article was submitted to Brain Disease Mechanisms, a section of the journal Frontiers in Molecular Neuroscience |
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| Title | Electrophysiological Properties of Human Cortical Organoids: Current State of the Art and Future Directions |
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