α-Amyrin and β-Amyrin Isolated from Celastrus hindsii Leaves and Their Antioxidant, Anti-Xanthine Oxidase, and Anti-Tyrosinase Potentials

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 23; p. 7248
• Main Authors: Viet, Tran Duc, Xuan, Tran Dang, Anh, La Hoang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 29.11.2021; MDPI
• Subjects: Antioxidants; Antioxidants - pharmacology; Callionymus hindsii; Cancer; Carbon-13 Magnetic Resonance Spectroscopy; Celastrus; Celastrus - chemistry; Chromatography; Chronic illnesses; Column chromatography; Enzyme Inhibitors - pharmacology; ESI-MS; Flavonoids; Gas chromatography; Gas Chromatography-Mass Spectrometry; GC-MS; Gout; Herbal medicine; Hyperpigmentation; Inflammation; Ionization; Mass spectrometry; Mass spectroscopy; Medicinal plants; Molecular weight; Monophenol Monooxygenase - antagonists & inhibitors; Monophenol Monooxygenase - metabolism; NMR; Nuclear magnetic resonance; Oleanolic Acid - analogs & derivatives; Oleanolic Acid - chemistry; Oleanolic Acid - isolation & purification; Oxidative stress; Pentacyclic Triterpenes - chemistry; Pentacyclic Triterpenes - isolation & purification; Plant Leaves - chemistry; Proton Magnetic Resonance Spectroscopy; Rheumatism; Scientific imaging; Skin; Spectrometry, Mass, Electrospray Ionization; Spectroscopy; Sulfonic acid; Tyrosinase; Uric acid; Xanthine oxidase; Xanthine Oxidase - antagonists & inhibitors; Xanthine Oxidase - metabolism; α-amyrin; β-amyrin; Vietnam; GC-MS; anti-xanthine oxidase; NMR; antioxidant; chromatography; anti-tyrosinase; ESI-MS; β-amyrin; α-amyrin
• DOI: 10.3390/molecules26237248
• ISSN: 1420-3049

is a popular medicinal plant in Vietnam and Southeast Asian countries as well as in South America. In this study, an amount of 12.05 g of an α-amyrin and β-amyrin mixture was isolated from (10.75 g/kg dry weight) by column chromatography applying different solvent systems to obtain maximum efficiency. α-Amyrin and β-amyrin were then confirmed by gas chromatography-mass spectrometry (GC-MS), electrospray ionization-mass spectrometry (ESI-MS), and nuclear magnetic resonance (NMR). The antioxidant activities of the α-amyrin and β-amyrin mixture were determined via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,20-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays with IC of 125.55 and 155.28 µg/mL, respectively. The mixture exhibited a high potential for preventing gout by inhibiting a relevant key enzyme, xanthine oxidase (XO) (IC = 258.22 µg/mL). Additionally, an important enzyme in skin hyperpigmentation, tyrosinase, was suppressed by the α-amyrin and β-amyrin mixture (IC = 178.85 µg/mL). This study showed that is an abundant source for the isolation of α-amyrin and β-amyrin. Furthermore, this was the first study indicating that α-amyrin and β-amyrin mixture are promising in future therapies for gout and skin hyperpigmentation.