S5, a Withanolide Isolated from Physalis Pubescens L., Induces G2/M Cell Cycle Arrest via the EGFR/P38 Pathway in Human Melanoma A375 Cells

• Published in: Molecules (Basel, Switzerland) Vol. 23; no. 12; p. 3175
• Main Authors: Fan, Yuqi, Mao, Yiwei, Cao, Shijie, Xia, Guiyang, Zhang, Qiang, Zhang, Hongyang, Qiu, Feng, Kang, Ning
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.12.2018; MDPI
• Subjects: Antineoplastic Agents, Phytogenic - chemistry; Antineoplastic Agents, Phytogenic - isolation & purification; Antineoplastic Agents, Phytogenic - pharmacology; Apoptosis; Autophagy; Binding sites; Cell cycle; Cell growth; Cell Line, Tumor; Cell Proliferation - drug effects; Chemotherapy; Cyclin-dependent kinases; EGFR; ErbB Receptors - metabolism; Flow cytometry; G2 Phase Cell Cycle Checkpoints - drug effects; G2/M arrest; Homeostasis; Humans; Kidney cancer; Kinases; Medical prognosis; Melanoma; Molecular Structure; P38; p38 Mitogen-Activated Protein Kinases - metabolism; Physalis - chemistry; Proteins; Signal transduction; Signal Transduction - drug effects; Skin cancer; Withanolides - chemistry; Withanolides - isolation & purification; Withanolides - pharmacology; melanoma; S5; P38; G2/M arrest; EGFR

S5 is a withanolide natural product isolated from Physalis pubescens L. Our previous experimental studies found that it has significant antitumor activity on renal cell carcinoma. In the present study, the anti-melanoma effect of S5 and the related molecular mechanism was first investigated. It was...