Sensitive detection of endocrine disrupters using ionic liquid – Single walled carbon nanotubes modified screen-printed based biosensors

Simple and low cost biosensor based on screen-printed electrode for sensitive detection of some alkylphenols was developed, by entrapment of HRP in a nanocomposite gel based on single-walled carbon nanotubes (SWCNTs) and 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM][PF6]) ionic liquid. Ram...

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Published inTalanta (Oxford) Vol. 85; no. 4; pp. 2007 - 2013
Main Authors Gurban, Ana-Maria, Rotariu, Lucian, Baibarac, Mihaela, Baltog, Ioan, Bala, Camelia
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 30.09.2011
Elsevier
Subjects
Alkylphenols
Analytical chemistry
Biological and medical sciences
Biosensing Techniques - methods
Biosensors
Biotechnology
Chemistry
Dielectric Spectroscopy
Electrochemistry
Electrodes
Endocrine Disruptors - analysis
Endocrine Disruptors - chemistry
Enzyme
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General, instrumentation
Imidazoles - chemistry
Ionic liquid
Ionic Liquids - chemistry
Methods. Procedures. Technologies
Nanotubes, Carbon - chemistry
Phenols - analysis
Phenols - chemistry
Printing
Screen-printed electrode
Single-walled carbon nanotubes
Spectrometric and optical methods
Spectroscopy, Fourier Transform Infrared
Spectrum Analysis, Raman
Various methods and equipments
Screen-printed electrode
Enzyme
Single-walled carbon nanotubes
Alkylphenols
Ionic liquid
Fourier transform spectroscopy
Hydrogen peroxide
Stability
Carbon nanotubes
Chemical sensor
Infrared spectrometry
Electrocatalysis
Reduction
Sensitivity
Response time
Biosensor
Detection limit
Nanocomposite
Raman spectrometry
Detection
Electrochemical impedance spectroscopy
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Abstract Simple and low cost biosensor based on screen-printed electrode for sensitive detection of some alkylphenols was developed, by entrapment of HRP in a nanocomposite gel based on single-walled carbon nanotubes (SWCNTs) and 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM][PF6]) ionic liquid. Raman and FTIR spectroscopy, CV and EIS studies demonstrate the interaction between SWCNTs and ionic liquid. The nanocomposite gel, SWCNT–[BMIM][PF6] provides to the modified sensor a considerable enhanced electrocatalytic activity toward hydrogen peroxide reduction. The HRP based biosensor exhibits high sensitivity and good stability, allowing a detection of the alkylphenols at an applied potential of −0.2V vs. Ag/AgCl, in linear range from 5.5 to 97.7μM for 4-t-octylphenol and respectively, between 5.5 and 140μM for 4-n-nonylphenol, with a response time of about 5s. The detection limit was 1.1μM for 4-t-octylphenol, and respectively 0.4μM for 4-n-nonylphenol (S/N=3).
AbstractList Simple and low cost biosensor based on screen-printed electrode for sensitive detection of some alkylphenols was developed, by entrapment of HRP in a nanocomposite gel based on single-walled carbon nanotubes (SWCNTs) and 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM][PF(6)]) ionic liquid. Raman and FTIR spectroscopy, CV and EIS studies demonstrate the interaction between SWCNTs and ionic liquid. The nanocomposite gel, SWCNT-[BMIM][PF(6)] provides to the modified sensor a considerable enhanced electrocatalytic activity toward hydrogen peroxide reduction. The HRP based biosensor exhibits high sensitivity and good stability, allowing a detection of the alkylphenols at an applied potential of -0.2V vs. Ag/AgCl, in linear range from 5.5 to 97.7 μM for 4-t-octylphenol and respectively, between 5.5 and 140 μM for 4-n-nonylphenol, with a response time of about 5s. The detection limit was 1.1 μM for 4-t-octylphenol, and respectively 0.4 μM for 4-n-nonylphenol (S/N=3).
Simple and low cost biosensor based on screen-printed electrode for sensitive detection of some alkylphenols was developed, by entrapment of HRP in a nanocomposite gel based on single-walled carbon nanotubes (SWCNTs) and 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM][PF6]) ionic liquid. Raman and FTIR spectroscopy, CV and EIS studies demonstrate the interaction between SWCNTs and ionic liquid. The nanocomposite gel, SWCNT–[BMIM][PF6] provides to the modified sensor a considerable enhanced electrocatalytic activity toward hydrogen peroxide reduction. The HRP based biosensor exhibits high sensitivity and good stability, allowing a detection of the alkylphenols at an applied potential of −0.2V vs. Ag/AgCl, in linear range from 5.5 to 97.7μM for 4-t-octylphenol and respectively, between 5.5 and 140μM for 4-n-nonylphenol, with a response time of about 5s. The detection limit was 1.1μM for 4-t-octylphenol, and respectively 0.4μM for 4-n-nonylphenol (S/N=3).
Simple and low cost biosensor based on screen-printed electrode for sensitive detection of some alkylphenols was developed, by entrapment of HRP in a nanocomposite gel based on single-walled carbon nanotubes (SWCNTs) and 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM][PF sub(6]) ionic liquid. Raman and FTIR spectroscopy, CV and EIS studies demonstrate the interaction between SWCNTs and ionic liquid. The nanocomposite gel, SWCNT-[BMIM][PF) sub(6)] provides to the modified sensor a considerable enhanced electrocatalytic activity toward hydrogen peroxide reduction. The HRP based biosensor exhibits high sensitivity and good stability, allowing a detection of the alkylphenols at an applied potential of -0.2 V vs. Ag/AgCl, in linear range from 5.5 to 97.7 mu M for 4-t-octylphenol and respectively, between 5.5 and 140 mu M for 4-n-nonylphenol, with a response time of about 5 s. The detection limit was 1.1 mu M for 4-t-octylphenol, and respectively 0.4 mu M for 4-n-nonylphenol (S/N = 3).
Author Bala, Camelia
Gurban, Ana-Maria
Baibarac, Mihaela
Rotariu, Lucian
Baltog, Ioan
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Issue 4
Keywords Screen-printed electrode
Enzyme
Single-walled carbon nanotubes
Alkylphenols
Ionic liquid
Fourier transform spectroscopy
Hydrogen peroxide
Stability
Carbon nanotubes
Chemical sensor
Infrared spectrometry
Electrocatalysis
Reduction
Sensitivity
Response time
Biosensor
Detection limit
Nanocomposite
Raman spectrometry
Detection
Electrochemical impedance spectroscopy
Language English
License CC BY 4.0
Copyright © 2011 Elsevier B.V. All rights reserved.
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Snippet Simple and low cost biosensor based on screen-printed electrode for sensitive detection of some alkylphenols was developed, by entrapment of HRP in a...
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SubjectTerms Alkylphenols
Analytical chemistry
Biological and medical sciences
Biosensing Techniques - methods
Biosensors
Biotechnology
Chemistry
Dielectric Spectroscopy
Electrochemistry
Electrodes
Endocrine Disruptors - analysis
Endocrine Disruptors - chemistry
Enzyme
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General, instrumentation
Imidazoles - chemistry
Ionic liquid
Ionic Liquids - chemistry
Methods. Procedures. Technologies
Nanotubes, Carbon - chemistry
Phenols - analysis
Phenols - chemistry
Printing
Screen-printed electrode
Single-walled carbon nanotubes
Spectrometric and optical methods
Spectroscopy, Fourier Transform Infrared
Spectrum Analysis, Raman
Various methods and equipments
Title Sensitive detection of endocrine disrupters using ionic liquid – Single walled carbon nanotubes modified screen-printed based biosensors
URI https://dx.doi.org/10.1016/j.talanta.2011.07.045
https://www.ncbi.nlm.nih.gov/pubmed/21872052
https://search.proquest.com/docview/1221133858
https://search.proquest.com/docview/885906529
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