Prebiotic Potential of Oligosaccharides Obtained by Acid Hydrolysis of α-(1→3)-Glucan from Laetiporus sulphureus : A Pilot Study

• Published in: Molecules (Basel, Switzerland) Vol. 25; no. 23; p. 5542
• Main Authors: Wiater, Adrian, Waśko, Adam, Adamczyk, Paulina, Gustaw, Klaudia, Pleszczyńska, Małgorzata, Wlizło, Kamila, Skowronek, Marcin, Tomczyk, Michał, Szczodrak, Janusz
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 26.11.2020; MDPI
• Subjects: Bacteria; Biological activity; Cardiovascular disease; Chromatography, High Pressure Liquid; Colorectal cancer; Communication; Degree of polymerization; Fermentation; Fruit bodies; fruiting bodies; Fungal Polysaccharides - chemistry; Glucan; Glucans - chemistry; Glucose; High-performance liquid chromatography; Humans; Hydrolysis; Intestine; Lactobacillus; Laetiporus sulphureus; Liquid chromatography; Metabolic syndrome; Microbiomes; Microbiota; Oligosaccharides; Oligosaccharides - chemistry; Polymerization; Polyporales - chemistry; prebiotic; Prebiotics; Probiotics; Strains (organisms); α-(1→3)-glucan; α-(1→3)-glucooligosaccharides; α-(1→3)-glucan; α-(1→3)-glucooligosaccharides; Laetiporus sulphureus; prebiotic; fruiting bodies
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules25235542

Increasing knowledge of the role of the intestinal microbiome in human health and well-being has resulted in increased interest in prebiotics, mainly oligosaccharides of various origins. To date, there are no reports in the literature on the prebiotic properties of oligosaccharides produced by the hydrolysis of pure fungal α-(1→3)-glucan. The aim of this study was to prepare α-(1→3)-glucooligosaccharides (α-(1→3)-GOS) and to perform initial evaluation of their prebiotic potential. The oligosaccharides were obtained by acid hydrolysis of α-(1→3)-glucan isolated from the fruiting bodies of and then, characterized by HPLC. Fermentation of α-(1→3)-GOS and reference prebiotics was compared in in vitro pure cultures of , , and enteric bacterial strains. A mixture of α-(1→3)-GOS, notably with a degree of polymerization of 2 to 9, was obtained. The hydrolysate was utilized for growth by most of the strains tested and showed a strong bifidogenic effect, but did not promote the growth of and . α-(1→3)-GOS proved to be effective in the selective stimulation of beneficial bacteria and can be further tested to determine their prebiotic functionality.