Low-temperature enhances production of severe fever with thrombocytopenia syndrome virus virus-like particles

• Published in: Applied microbiology and biotechnology Vol. 109; no. 1; p. 56
• Main Authors: Loop, Isabelle, Ke, Yuan-Dun, Chen, Wei-June, Tsai, Kun-Hsien, Hsu, Wei-Li, Fan, Yi-Chin
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 03.03.2025; Springer Nature B.V
• Subjects: Animals; Antigens; Antigens, Viral - genetics; Antigens, Viral - immunology; Assembly; Biomedical and Life Sciences; Biotechnological Products and Process Engineering; Biotechnology; Centrifugation; Chlorocebus aethiops; Cold Temperature; COS Cells; density gradient centrifugation; Epitopes; Epitopes - immunology; Fever; genotype; Genotypes; Glycoproteins; Glycoproteins - genetics; Glycoproteins - immunology; HEK293 Cells; Huaiyangshan banyangvirus; Humans; Life Sciences; Low temperature; Microbial Genetics and Genomics; Microbiology; monitoring; Monoclonal antibodies; nucleocapsid proteins; Nucleocapsids; Phlebovirus - genetics; Phlebovirus - immunology; plasmids; Proteins; RNA; Serology; severe fever with thrombocytopenia syndrome; Severe Fever with Thrombocytopenia Syndrome - virology; sucrose; Surveillance; Thrombocytopenia; Transmission electron microscopy; Vaccine development; Vaccines; Vaccines, Virus-Like Particle - immunology; Viral Proteins - genetics; virion; Virions; Virus-like particles; Viruses; Zoonoses; Genotype; Severe fever with thrombocytopenia syndrome virus; Virus-like particle; Low-temperature cultivation; Glycoprotein; Mammalian cells

Tick-borne severe fever with thrombocytopenia syndrome (SFTS) is an emerging zoonotic disease caused by the SFTS virus (SFTSV). Serological assays based on the nucleocapsid protein and partial glycoprotein of this virus have been used for detecting SFTSV infections in humans and animals. However, wh...