Robust Response to Plum pox virus Infection via Plant Biotechnology

• Published in: Genes Vol. 12; no. 6; p. 816
• Main Authors: Ravelonandro, Michel, Briard, Pascal, Scorza, Ralph, Callahan, Ann, Zagrai, Ioan, Kundu, Jiban K., Dardick, Chris
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 27.05.2021; MDPI
• Subjects: Biotechnology; Capsids; Cloning; Coat protein; DNA methylation; Epigenetics; gene construct; Genes; Genetic engineering; Genomes; hairpin; Hybridization; Infections; Life Sciences; Phytopathology and phytopharmacy; Plant breeding; Plant resistance; Plant virus diseases; Plum pox; Plum pox virus; Prunus domestica; R&D; Research & development; resistance; RNAi; Transgenic plants; Vegetal Biology; Viruses; arbre fruitier à noyau; RNAi; Plum pox virus; Prunus domestica; pathologie végétale; fruit; hairpin; gene construct; resistance; virus phytopathogène; virologie végétale

Our goal was to target silencing of the Plum pox virus coat protein (PPV CP) gene independently expressed in plants. Clone C-2 is a transgenic plum expressing CP. We introduced and verified, in planta, the effects of the inverse repeat of CP sequence split by a hairpin (IRSH) that was characterized...