Single cell immunoprofile of synovial fluid in rheumatoid arthritis with TNF/JAK inhibitor treatment

• Published in: Nature communications Vol. 16; no. 1; pp. 2152 - 23
• Main Authors: Xia, Xuyang, He, Chenjia, Xue, Zhinan, Wang, Yuelan, Qin, Yun, Ren, Zhixiang, Huang, Yupeng, Luo, Han, Chen, Hai-Ning, Zhang, Wei-Han, Huang, Li-Bin, Shi, Yunying, Bai, Yangjuan, Cai, Bei, Wang, Lanlan, Zhang, Feng, Qian, Maoxiang, Zhang, Wei, Shu, Yang, Yin, Geng, Xu, Heng, Xie, Qibing
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 04.03.2025; Nature Publishing Group; Nature Portfolio
• Subjects: 13/31; 13/51; 38/77; 45/47; 45/91; 631/208/514/1949; 631/250/1619/554/1898; 631/250/2502; 631/250/2504/342; 692/699/1670/498; 96/47; Adalimumab - pharmacology; Adalimumab - therapeutic use; Animal models; Animals; Arthritis; Arthritis, Experimental - drug therapy; Arthritis, Experimental - immunology; Arthritis, Rheumatoid - drug therapy; Arthritis, Rheumatoid - genetics; Arthritis, Rheumatoid - immunology; Arthritis, Rheumatoid - metabolism; Arthritis, Rheumatoid - pathology; CD4 antigen; CD4-Positive T-Lymphocytes - drug effects; CD4-Positive T-Lymphocytes - immunology; CD4-Positive T-Lymphocytes - metabolism; Cell culture; Cell interactions; CXCL13 protein; Disease Models, Animal; Female; Gene expression; Gene sequencing; Humanities and Social Sciences; Humans; Immune system; Inflammation; Janus Kinase Inhibitors - pharmacology; Janus Kinase Inhibitors - therapeutic use; Lymphocytes; Lymphocytes T; Macrophages; Macrophages - drug effects; Macrophages - immunology; Macrophages - metabolism; Male; Mice; Middle Aged; Monoclonal antibodies; multidisciplinary; Piperidines; Pyrimidines - pharmacology; Pyrimidines - therapeutic use; Rheumatoid arthritis; Science; Science (multidisciplinary); Single-Cell Analysis - methods; Stat1 protein; Synovial fluid; Synovial Fluid - cytology; Synovial Fluid - drug effects; Synovial Fluid - immunology; Synoviocytes; Synoviocytes - drug effects; Synoviocytes - immunology; Synoviocytes - metabolism; Tumor Necrosis Factor Inhibitors - pharmacology; Tumor Necrosis Factor Inhibitors - therapeutic use; Tumor Necrosis Factor-alpha - antagonists & inhibitors

Numerous patients with rheumatoid arthritis (RA) manifest severe syndromes, including elevated synovial fluid volumes (SF) with abundant immune cells, which can be controlled by TNF/JAK inhibitors. Here, we apply single-cell RNA sequencing (scRNA-seq) and subsequent validations in SF from RA patient...