Improved efficacy of SARS-CoV-2 isolation from COVID-19 clinical specimens using VeroE6 cells overexpressing TMPRSS2 and human ACE2

The cell culture-based isolation of novel coronavirus SARS-CoV-2 from clinical specimens obtained from patients with suspected COVID-19 is important not only for laboratory diagnosis but also for obtaining live virus to characterize emerging variants. Previous studies report that monkey kidney-deriv...

Full description

Saved in:
Bibliographic Details
Published inScientific reports Vol. 14; no. 1; pp. 24858 - 12
Main Authors Kinoshita, Hitomi, Yamamoto, Tsukasa, Kuroda, Yudai, Inoue, Yusuke, Miyazaki, Kaya, Ohmagari, Norio, Tokita, Daisuke, Nguyen, Phu Hoang Anh, Yamada, Souichi, Harada, Shizuko, Kanno, Takayuki, Takahashi, Kenichiro, Saito, Masumichi, Shirato, Kazuya, Takayama, Ikuyo, Watanabe, Shinji, Saito, Tomoya, Ebihara, Hideki, Suzuki, Tadaki, Maeda, Ken, Fukushi, Shuetsu
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 22.10.2024
Nature Publishing Group
Nature Portfolio
Subjects
631/326/596
631/326/596/4130
ACE2
Angiotensin-converting enzyme 2
Angiotensin-Converting Enzyme 2 - genetics
Angiotensin-Converting Enzyme 2 - metabolism
Animals
Cell culture
Chlorocebus aethiops
Coronaviruses
COVID-19
COVID-19 - metabolism
COVID-19 - virology
Humanities and Social Sciences
Humans
Infectivity
multidisciplinary
Neutralization
SARS-CoV-2
SARS-CoV-2 - genetics
SARS-CoV-2 - isolation & purification
SARS-CoV-2 - metabolism
Science
Science (multidisciplinary)
Serine Endopeptidases - genetics
Serine Endopeptidases - metabolism
Severe acute respiratory syndrome coronavirus 2
Stomatitis
Titration
TMPRSS2
Vero Cells
Viral Load
Virus isolation
ACE2
TMPRSS2
SARS-CoV-2
Virus isolation
Online AccessGet full text

Cover

Abstract The cell culture-based isolation of novel coronavirus SARS-CoV-2 from clinical specimens obtained from patients with suspected COVID-19 is important not only for laboratory diagnosis but also for obtaining live virus to characterize emerging variants. Previous studies report that monkey kidney-derived VeroE6/TMPRSS2 cells allow efficient isolation of SARS-CoV-2 from clinical specimens because these cells show stable expression of the receptor molecule monkey ACE2 and the serine-protease TMPRSS2. Here, we demonstrated that VeroE6 cells overexpressing human ACE2 and TMPRSS2 (Vero E6-TMPRSS2-T2A-ACE2 cells) are superior to VeroE6/TMPRSS2 for isolating SARS-CoV-2 from clinical specimens. These cells showed a 1.6-fold increase in efficiency in SARS-CoV-2 isolation, and were particularly effective for clinical specimens with a relatively low viral load (< 10 6 copies/mL). When using vesicular stomatitis virus (VSV) pseudoviruses (VSV/SARS-2pv) bearing the spike proteins of all of the tested SARS-CoV-2 strains, Vero E6-TMPRSS2-T2A-ACE2 cells showed a 2– to fourfold increase in infectivity. Furthermore, the results of virus titration and neutralization antibody assays using Vero E6-TMPRSS2-T2A-ACE2 cells were different from those using VeroE6/TMPRSS2, highlighting the importance of selecting appropriate cell culture systems to determine SARS-CoV-2 infectivity.
AbstractList The cell culture-based isolation of novel coronavirus SARS-CoV-2 from clinical specimens obtained from patients with suspected COVID-19 is important not only for laboratory diagnosis but also for obtaining live virus to characterize emerging variants. Previous studies report that monkey kidney-derived VeroE6/TMPRSS2 cells allow efficient isolation of SARS-CoV-2 from clinical specimens because these cells show stable expression of the receptor molecule monkey ACE2 and the serine-protease TMPRSS2. Here, we demonstrated that VeroE6 cells overexpressing human ACE2 and TMPRSS2 (Vero E6-TMPRSS2-T2A-ACE2 cells) are superior to VeroE6/TMPRSS2 for isolating SARS-CoV-2 from clinical specimens. These cells showed a 1.6-fold increase in efficiency in SARS-CoV-2 isolation, and were particularly effective for clinical specimens with a relatively low viral load (< 106 copies/mL). When using vesicular stomatitis virus (VSV) pseudoviruses (VSV/SARS-2pv) bearing the spike proteins of all of the tested SARS-CoV-2 strains, Vero E6-TMPRSS2-T2A-ACE2 cells showed a 2– to fourfold increase in infectivity. Furthermore, the results of virus titration and neutralization antibody assays using Vero E6-TMPRSS2-T2A-ACE2 cells were different from those using VeroE6/TMPRSS2, highlighting the importance of selecting appropriate cell culture systems to determine SARS-CoV-2 infectivity.
The cell culture-based isolation of novel coronavirus SARS-CoV-2 from clinical specimens obtained from patients with suspected COVID-19 is important not only for laboratory diagnosis but also for obtaining live virus to characterize emerging variants. Previous studies report that monkey kidney-derived VeroE6/TMPRSS2 cells allow efficient isolation of SARS-CoV-2 from clinical specimens because these cells show stable expression of the receptor molecule monkey ACE2 and the serine-protease TMPRSS2. Here, we demonstrated that VeroE6 cells overexpressing human ACE2 and TMPRSS2 (Vero E6-TMPRSS2-T2A-ACE2 cells) are superior to VeroE6/TMPRSS2 for isolating SARS-CoV-2 from clinical specimens. These cells showed a 1.6-fold increase in efficiency in SARS-CoV-2 isolation, and were particularly effective for clinical specimens with a relatively low viral load (< 10 copies/mL). When using vesicular stomatitis virus (VSV) pseudoviruses (VSV/SARS-2pv) bearing the spike proteins of all of the tested SARS-CoV-2 strains, Vero E6-TMPRSS2-T2A-ACE2 cells showed a 2- to fourfold increase in infectivity. Furthermore, the results of virus titration and neutralization antibody assays using Vero E6-TMPRSS2-T2A-ACE2 cells were different from those using VeroE6/TMPRSS2, highlighting the importance of selecting appropriate cell culture systems to determine SARS-CoV-2 infectivity.
The cell culture-based isolation of novel coronavirus SARS-CoV-2 from clinical specimens obtained from patients with suspected COVID-19 is important not only for laboratory diagnosis but also for obtaining live virus to characterize emerging variants. Previous studies report that monkey kidney-derived VeroE6/TMPRSS2 cells allow efficient isolation of SARS-CoV-2 from clinical specimens because these cells show stable expression of the receptor molecule monkey ACE2 and the serine-protease TMPRSS2. Here, we demonstrated that VeroE6 cells overexpressing human ACE2 and TMPRSS2 (Vero E6-TMPRSS2-T2A-ACE2 cells) are superior to VeroE6/TMPRSS2 for isolating SARS-CoV-2 from clinical specimens. These cells showed a 1.6-fold increase in efficiency in SARS-CoV-2 isolation, and were particularly effective for clinical specimens with a relatively low viral load (< 106 copies/mL). When using vesicular stomatitis virus (VSV) pseudoviruses (VSV/SARS-2pv) bearing the spike proteins of all of the tested SARS-CoV-2 strains, Vero E6-TMPRSS2-T2A-ACE2 cells showed a 2- to fourfold increase in infectivity. Furthermore, the results of virus titration and neutralization antibody assays using Vero E6-TMPRSS2-T2A-ACE2 cells were different from those using VeroE6/TMPRSS2, highlighting the importance of selecting appropriate cell culture systems to determine SARS-CoV-2 infectivity.The cell culture-based isolation of novel coronavirus SARS-CoV-2 from clinical specimens obtained from patients with suspected COVID-19 is important not only for laboratory diagnosis but also for obtaining live virus to characterize emerging variants. Previous studies report that monkey kidney-derived VeroE6/TMPRSS2 cells allow efficient isolation of SARS-CoV-2 from clinical specimens because these cells show stable expression of the receptor molecule monkey ACE2 and the serine-protease TMPRSS2. Here, we demonstrated that VeroE6 cells overexpressing human ACE2 and TMPRSS2 (Vero E6-TMPRSS2-T2A-ACE2 cells) are superior to VeroE6/TMPRSS2 for isolating SARS-CoV-2 from clinical specimens. These cells showed a 1.6-fold increase in efficiency in SARS-CoV-2 isolation, and were particularly effective for clinical specimens with a relatively low viral load (< 106 copies/mL). When using vesicular stomatitis virus (VSV) pseudoviruses (VSV/SARS-2pv) bearing the spike proteins of all of the tested SARS-CoV-2 strains, Vero E6-TMPRSS2-T2A-ACE2 cells showed a 2- to fourfold increase in infectivity. Furthermore, the results of virus titration and neutralization antibody assays using Vero E6-TMPRSS2-T2A-ACE2 cells were different from those using VeroE6/TMPRSS2, highlighting the importance of selecting appropriate cell culture systems to determine SARS-CoV-2 infectivity.
Abstract The cell culture-based isolation of novel coronavirus SARS-CoV-2 from clinical specimens obtained from patients with suspected COVID-19 is important not only for laboratory diagnosis but also for obtaining live virus to characterize emerging variants. Previous studies report that monkey kidney-derived VeroE6/TMPRSS2 cells allow efficient isolation of SARS-CoV-2 from clinical specimens because these cells show stable expression of the receptor molecule monkey ACE2 and the serine-protease TMPRSS2. Here, we demonstrated that VeroE6 cells overexpressing human ACE2 and TMPRSS2 (Vero E6-TMPRSS2-T2A-ACE2 cells) are superior to VeroE6/TMPRSS2 for isolating SARS-CoV-2 from clinical specimens. These cells showed a 1.6-fold increase in efficiency in SARS-CoV-2 isolation, and were particularly effective for clinical specimens with a relatively low viral load (< 106 copies/mL). When using vesicular stomatitis virus (VSV) pseudoviruses (VSV/SARS-2pv) bearing the spike proteins of all of the tested SARS-CoV-2 strains, Vero E6-TMPRSS2-T2A-ACE2 cells showed a 2– to fourfold increase in infectivity. Furthermore, the results of virus titration and neutralization antibody assays using Vero E6-TMPRSS2-T2A-ACE2 cells were different from those using VeroE6/TMPRSS2, highlighting the importance of selecting appropriate cell culture systems to determine SARS-CoV-2 infectivity.
The cell culture-based isolation of novel coronavirus SARS-CoV-2 from clinical specimens obtained from patients with suspected COVID-19 is important not only for laboratory diagnosis but also for obtaining live virus to characterize emerging variants. Previous studies report that monkey kidney-derived VeroE6/TMPRSS2 cells allow efficient isolation of SARS-CoV-2 from clinical specimens because these cells show stable expression of the receptor molecule monkey ACE2 and the serine-protease TMPRSS2. Here, we demonstrated that VeroE6 cells overexpressing human ACE2 and TMPRSS2 (Vero E6-TMPRSS2-T2A-ACE2 cells) are superior to VeroE6/TMPRSS2 for isolating SARS-CoV-2 from clinical specimens. These cells showed a 1.6-fold increase in efficiency in SARS-CoV-2 isolation, and were particularly effective for clinical specimens with a relatively low viral load (< 10 6 copies/mL). When using vesicular stomatitis virus (VSV) pseudoviruses (VSV/SARS-2pv) bearing the spike proteins of all of the tested SARS-CoV-2 strains, Vero E6-TMPRSS2-T2A-ACE2 cells showed a 2– to fourfold increase in infectivity. Furthermore, the results of virus titration and neutralization antibody assays using Vero E6-TMPRSS2-T2A-ACE2 cells were different from those using VeroE6/TMPRSS2, highlighting the importance of selecting appropriate cell culture systems to determine SARS-CoV-2 infectivity.
ArticleNumber 24858
Author Kuroda, Yudai
Harada, Shizuko
Saito, Tomoya
Suzuki, Tadaki
Ebihara, Hideki
Kanno, Takayuki
Watanabe, Shinji
Kinoshita, Hitomi
Ohmagari, Norio
Yamada, Souichi
Yamamoto, Tsukasa
Inoue, Yusuke
Shirato, Kazuya
Takayama, Ikuyo
Fukushi, Shuetsu
Nguyen, Phu Hoang Anh
Miyazaki, Kaya
Tokita, Daisuke
Takahashi, Kenichiro
Saito, Masumichi
Maeda, Ken
Author_xml – sequence: 1
  givenname: Hitomi
  surname: Kinoshita
  fullname: Kinoshita, Hitomi
  organization: Department of Virology I, National Institute of Infectious Diseases
– sequence: 2
  givenname: Tsukasa
  surname: Yamamoto
  fullname: Yamamoto, Tsukasa
  organization: Department of Veterinary Science, National Institute of Infectious Diseases
– sequence: 3
  givenname: Yudai
  surname: Kuroda
  fullname: Kuroda, Yudai
  organization: Department of Veterinary Science, National Institute of Infectious Diseases
– sequence: 4
  givenname: Yusuke
  surname: Inoue
  fullname: Inoue, Yusuke
  organization: Department of Veterinary Science, National Institute of Infectious Diseases
– sequence: 5
  givenname: Kaya
  surname: Miyazaki
  fullname: Miyazaki, Kaya
  organization: Research Center for Influenza and Respiratory Viruses, National Institute of Infectious Diseases
– sequence: 6
  givenname: Norio
  surname: Ohmagari
  fullname: Ohmagari, Norio
  organization: Disease Control and Prevention Center, National Center for Global Health and Medicine
– sequence: 7
  givenname: Daisuke
  surname: Tokita
  fullname: Tokita, Daisuke
  organization: Center for Clinical Sciences, National Center for Global Health and Medicine
– sequence: 8
  givenname: Phu Hoang Anh
  surname: Nguyen
  fullname: Nguyen, Phu Hoang Anh
  organization: Department of Virology I, National Institute of Infectious Diseases
– sequence: 9
  givenname: Souichi
  surname: Yamada
  fullname: Yamada, Souichi
  organization: Department of Virology I, National Institute of Infectious Diseases
– sequence: 10
  givenname: Shizuko
  surname: Harada
  fullname: Harada, Shizuko
  organization: Department of Virology I, National Institute of Infectious Diseases
– sequence: 11
  givenname: Takayuki
  surname: Kanno
  fullname: Kanno, Takayuki
  organization: Department of Pathology, National Institute of Infectious Diseases
– sequence: 12
  givenname: Kenichiro
  surname: Takahashi
  fullname: Takahashi, Kenichiro
  organization: Center for Emergency Preparedness and Response, National Institute of Infectious Diseases
– sequence: 13
  givenname: Masumichi
  surname: Saito
  fullname: Saito, Masumichi
  organization: Center for Emergency Preparedness and Response, National Institute of Infectious Diseases, Department of Virology II, National Institute of Infectious Diseases
– sequence: 14
  givenname: Kazuya
  surname: Shirato
  fullname: Shirato, Kazuya
  organization: Department of Virology III, National Institute of Infectious Diseases
– sequence: 15
  givenname: Ikuyo
  surname: Takayama
  fullname: Takayama, Ikuyo
  organization: Research Center for Influenza and Respiratory Viruses, National Institute of Infectious Diseases
– sequence: 16
  givenname: Shinji
  surname: Watanabe
  fullname: Watanabe, Shinji
  organization: Research Center for Influenza and Respiratory Viruses, National Institute of Infectious Diseases
– sequence: 17
  givenname: Tomoya
  surname: Saito
  fullname: Saito, Tomoya
  organization: Center for Emergency Preparedness and Response, National Institute of Infectious Diseases
– sequence: 18
  givenname: Hideki
  surname: Ebihara
  fullname: Ebihara, Hideki
  organization: Department of Virology I, National Institute of Infectious Diseases
– sequence: 19
  givenname: Tadaki
  surname: Suzuki
  fullname: Suzuki, Tadaki
  organization: Department of Pathology, National Institute of Infectious Diseases
– sequence: 20
  givenname: Ken
  surname: Maeda
  fullname: Maeda, Ken
  organization: Department of Veterinary Science, National Institute of Infectious Diseases
– sequence: 21
  givenname: Shuetsu
  surname: Fukushi
  fullname: Fukushi, Shuetsu
  email: fukushi@niid.go.jp
  organization: Department of Virology I, National Institute of Infectious Diseases
BackLink https://www.ncbi.nlm.nih.gov/pubmed/39438626$$D View this record in MEDLINE/PubMed
BookMark eNp9ks9v0zAUgCM0xMbYP8ABWeLCJeDfsU-oKgUqDQ2to1fLcZzOVWJndjOxM_84bjPGxgFfbL33-fPT83tZHPngbVG8RvA9gkR8SBQxKUqIaVmxHCjps-IEQ8pKTDA-enQ-Ls5S2sK8GJYUyRfFMZGUCI75SfFr2Q8x3NoG2LZ1Rps7EFqwml2uynlYlxi4FDq9c8GDNoYezC_Wy08lksB0zme-A2mwxvXWJzAm5zdgbWNYcGBs1yWQzdH-HKJNh9zVt--XqxUG2jfgeuy1B7P5Ar8qnre6S_bsfj8tfnxeXM2_lucXX5bz2XlpqMS7sjYNhFQyqGWtjZCYIyaMEAZyQipWWc5QZVhDG0FrYkktdCtohaRAxnLCyWmxnLxN0Fs1RNfreKeCduoQCHGjdNw501lls06TWgtDETWaSkEJNEwawXMtyGTXx8k1jHVvG2P9LuruifRpxrtrtQm3CiEqORcwG97dG2K4GW3aqd6lfde0t2FMiiAkK4wlqTL69h90G8boc68OFGFQVjRTbx6X9FDLn8_OAJ4AE0NK0bYPCIJqP1RqGiqVh0odhkrtrWS6lDLsNzb-ffs_t34DcM_L0A
Cites_doi 10.1038/s41586-020-2196-x
10.3390/v14020383
10.1002/rmv.2381
10.1128/mSphere.00401-17
10.1016/j.medj.2022.02.006
10.1128/JVI.01890-13
10.1038/s41586-020-2012-7
10.2807/1560-7917.ES.2020.25.32.2001483
10.1038/s41586-022-04411-y
10.1080/22221751.2021.2023329
10.1016/S1473-3099(24)00155-5
10.1016/j.virol.2022.05.004
10.1136/bmjresp-2020-000830
10.1099/vir.0.81749-0
10.1056/NEJMoa2008457
10.1056/NEJMc2206576
10.1016/j.chom.2022.09.018
10.1097/JS9.0000000000000521
10.1007/s10096-020-03913-9
10.7883/yoken.JJID.2020.061
10.1128/JVI.00649-15
10.1186/s12985-021-01490-7
10.1073/pnas.2002589117
10.1038/s41467-023-39890-8
10.1016/j.isci.2023.106634
10.1371/journal.pone.0289432
10.1038/s41586-022-04474-x
10.1038/s41564-020-0695-z
10.17504/protocols.io.bp2l6n26rgqe/v3
10.17504/protocols.io.betejeje
ContentType Journal Article
Copyright The Author(s) 2024
2024. The Author(s).
The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
The Author(s) 2024 2024
Copyright_xml – notice: The Author(s) 2024
– notice: 2024. The Author(s).
– notice: The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: The Author(s) 2024 2024
DBID C6C
AAYXX
CITATION
CGR
CUY
CVF
ECM
EIF
NPM
3V.
7X7
7XB
88A
88E
88I
8FE
8FH
8FI
8FJ
8FK
ABUWG
AEUYN
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
CCPQU
COVID
DWQXO
FYUFA
GHDGH
GNUQQ
HCIFZ
K9.
LK8
M0S
M1P
M2P
M7P
PHGZM
PHGZT
PIMPY
PJZUB
PKEHL
PPXIY
PQEST
PQGLB
PQQKQ
PQUKI
PRINS
Q9U
7X8
5PM
DOA
DOI 10.1038/s41598-024-75038-4
DatabaseName Springer Nature OA Free Journals (WRLC)
CrossRef
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
ProQuest Central (Corporate)
Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Biology Database (Alumni Edition)
Medical Database (Alumni Edition)
Science Database (Alumni Edition)
ProQuest SciTech Collection
ProQuest Natural Science Collection
Hospital Premium Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
ProQuest Central (Alumni)
ProQuest One Sustainability
ProQuest Central UK/Ireland
ProQuest Central Essentials
Biological Science Collection
ProQuest Central (New)
Natural Science Collection
ProQuest One Community College
Coronavirus Research Database
ProQuest Central
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
SciTech Premium Collection
ProQuest Health & Medical Complete (Alumni)
ProQuest Biological Science Collection
ProQuest Health & Medical Collection
PML(ProQuest Medical Library)
Science Database
Biological Science Database
ProQuest Central Premium
ProQuest One Academic (New)
Publicly Available Content Database
ProQuest Health & Medical Research Collection
ProQuest One Academic Middle East (New)
ProQuest One Health & Nursing
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Applied & Life Sciences
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
ProQuest Central Basic
MEDLINE - Academic
PubMed Central (Full Participant titles)
DOAJ Directory of Open Access Journals
DatabaseTitle CrossRef
MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
Publicly Available Content Database
ProQuest Central Student
ProQuest One Academic Middle East (New)
ProQuest Central Essentials
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest One Health & Nursing
ProQuest Natural Science Collection
ProQuest Central China
ProQuest Biology Journals (Alumni Edition)
ProQuest Central
ProQuest One Applied & Life Sciences
ProQuest One Sustainability
ProQuest Health & Medical Research Collection
Health Research Premium Collection
Health and Medicine Complete (Alumni Edition)
Natural Science Collection
ProQuest Central Korea
Health & Medical Research Collection
Biological Science Collection
ProQuest Central (New)
ProQuest Medical Library (Alumni)
ProQuest Science Journals (Alumni Edition)
ProQuest Biological Science Collection
ProQuest Central Basic
ProQuest Science Journals
ProQuest One Academic Eastern Edition
Coronavirus Research Database
ProQuest Hospital Collection
Health Research Premium Collection (Alumni)
Biological Science Database
ProQuest SciTech Collection
ProQuest Hospital Collection (Alumni)
ProQuest Health & Medical Complete
ProQuest Medical Library
ProQuest One Academic UKI Edition
ProQuest One Academic
ProQuest One Academic (New)
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList Publicly Available Content Database
MEDLINE
MEDLINE - Academic
Database_xml – sequence: 1
  dbid: C6C
  name: SpringerOpen Free (Free internet resource, activated by CARLI)
  url: http://www.springeropen.com/
  sourceTypes: Publisher
– sequence: 2
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 3
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 4
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
– sequence: 5
  dbid: BENPR
  name: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 2045-2322
EndPage 12
ExternalDocumentID oai_doaj_org_article_e57ea3ba8c414ca498430c59c864921c
PMC11496680
39438626
10_1038_s41598_024_75038_4
Genre Journal Article
GrantInformation_xml – fundername: Japan Agency for Medical Research and Development
  grantid: JP21fk0108615; JP22fk0108509
  funderid: http://dx.doi.org/10.13039/100009619
– fundername: Japan Agency for Medical Research and Development
  grantid: JP22fk0108509
– fundername: Japan Agency for Medical Research and Development
  grantid: JP21fk0108615
GroupedDBID 0R~
3V.
4.4
53G
5VS
7X7
88A
88E
88I
8FE
8FH
8FI
8FJ
AAFWJ
AAJSJ
AAKDD
ABDBF
ABUWG
ACGFS
ACSMW
ACUHS
ADBBV
ADRAZ
AENEX
AEUYN
AFKRA
AJTQC
ALIPV
ALMA_UNASSIGNED_HOLDINGS
AOIJS
AZQEC
BAWUL
BBNVY
BCNDV
BENPR
BHPHI
BPHCQ
BVXVI
C6C
CCPQU
DIK
DWQXO
EBD
EBLON
EBS
ESX
FYUFA
GNUQQ
GROUPED_DOAJ
GX1
HCIFZ
HH5
HMCUK
HYE
KQ8
LK8
M0L
M1P
M2P
M48
M7P
M~E
NAO
OK1
PIMPY
PQQKQ
PROAC
PSQYO
RNT
RNTTT
RPM
SNYQT
UKHRP
AASML
AAYXX
AFPKN
CITATION
PHGZM
PHGZT
CGR
CUY
CVF
ECM
EIF
NPM
PJZUB
PPXIY
PQGLB
7XB
8FK
AARCD
COVID
K9.
PKEHL
PQEST
PQUKI
PRINS
Q9U
7X8
5PM
PUEGO
ID FETCH-LOGICAL-c492t-bcd004950a9bac8926158c88c0633757e6517c5d4d84b3e3b8af8471981ce6363
IEDL.DBID M48
ISSN 2045-2322
IngestDate Wed Aug 27 01:31:45 EDT 2025
Thu Aug 21 18:31:01 EDT 2025
Fri Jul 11 03:00:02 EDT 2025
Wed Aug 13 04:55:07 EDT 2025
Mon Jul 21 05:28:24 EDT 2025
Tue Jul 01 03:23:17 EDT 2025
Fri Feb 21 02:37:42 EST 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 1
Keywords ACE2
TMPRSS2
SARS-CoV-2
Virus isolation
Language English
License 2024. The Author(s).
Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c492t-bcd004950a9bac8926158c88c0633757e6517c5d4d84b3e3b8af8471981ce6363
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
OpenAccessLink http://journals.scholarsportal.info/openUrl.xqy?doi=10.1038/s41598-024-75038-4
PMID 39438626
PQID 3119350974
PQPubID 2041939
PageCount 12
ParticipantIDs doaj_primary_oai_doaj_org_article_e57ea3ba8c414ca498430c59c864921c
pubmedcentral_primary_oai_pubmedcentral_nih_gov_11496680
proquest_miscellaneous_3119722937
proquest_journals_3119350974
pubmed_primary_39438626
crossref_primary_10_1038_s41598_024_75038_4
springer_journals_10_1038_s41598_024_75038_4
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 2024-10-22
PublicationDateYYYYMMDD 2024-10-22
PublicationDate_xml – month: 10
  year: 2024
  text: 2024-10-22
  day: 22
PublicationDecade 2020
PublicationPlace London
PublicationPlace_xml – name: London
– name: England
PublicationTitle Scientific reports
PublicationTitleAbbrev Sci Rep
PublicationTitleAlternate Sci Rep
PublicationYear 2024
Publisher Nature Publishing Group UK
Nature Publishing Group
Nature Portfolio
Publisher_xml – name: Nature Publishing Group UK
– name: Nature Publishing Group
– name: Nature Portfolio
References Zhou (CR1) 2020; 579
CR18
CR17
Tani (CR20) 2021; 18
CR16
Wolfel (CR29) 2020; 581
Bhat, Cao, Yin (CR34) 2022
Ren (CR11) 2006; 87
Cao (CR23) 2022; 30
Arons (CR25) 2020; 382
Yamada (CR13) 2021
Meng (CR24) 2022; 603
Shrestha, Foster, Rawlinson, Tedla, Bull (CR5) 2022; 32
Chen (CR31) 2023; 26
CR2
CR6
Matsuyama (CR12) 2020; 117
Wannigama (CR8) 2024
Rucker, Qin, Zhang (CR22) 2023; 18
CR9
Viana (CR3) 2022; 603
Yoshida (CR30) 2023; 74
Moriyama (CR28) 2023; 14
Singanayagam (CR26) 2020
Alfson (CR33) 2018
Shirato (CR10) 2020; 73
Cheng (CR7) 2023; 109
Shirato, Kawase, Matsuyama (CR21) 2013; 87
La Scola (CR27) 2020; 39
Hachmann (CR4) 2022; 387
Miyamoto (CR19) 2022; 3
Zhao (CR14) 2022; 11
Kumar (CR15) 2022; 572
Alfson (CR32) 2015; 89
KJ Alfson (75038_CR33) 2018
K Cheng (75038_CR7) 2023; 109
S Matsuyama (75038_CR12) 2020; 117
S Miyamoto (75038_CR19) 2022; 3
S Moriyama (75038_CR28) 2023; 14
LB Shrestha (75038_CR5) 2022; 32
MM Arons (75038_CR25) 2020; 382
NP Hachmann (75038_CR4) 2022; 387
I Yoshida (75038_CR30) 2023; 74
A Singanayagam (75038_CR26) 2020
B Meng (75038_CR24) 2022; 603
Y Cao (75038_CR23) 2022; 30
DY Chen (75038_CR31) 2023; 26
75038_CR6
75038_CR2
G Rucker (75038_CR22) 2023; 18
DL Wannigama (75038_CR8) 2024
75038_CR9
H Zhao (75038_CR14) 2022; 11
P Zhou (75038_CR1) 2020; 579
R Wolfel (75038_CR29) 2020; 581
75038_CR18
K Shirato (75038_CR21) 2013; 87
X Ren (75038_CR11) 2006; 87
75038_CR16
75038_CR17
R Viana (75038_CR3) 2022; 603
B La Scola (75038_CR27) 2020; 39
H Tani (75038_CR20) 2021; 18
KJ Alfson (75038_CR32) 2015; 89
S Yamada (75038_CR13) 2021
K Shirato (75038_CR10) 2020; 73
P Kumar (75038_CR15) 2022; 572
T Bhat (75038_CR34) 2022
References_xml – ident: CR18
– volume: 581
  start-page: 465
  year: 2020
  end-page: 469
  ident: CR29
  article-title: Virological assessment of hospitalized patients with COVID-2019
  publication-title: Nature
  doi: 10.1038/s41586-020-2196-x
– year: 2022
  ident: CR34
  article-title: Virus-like Particles: Measures and biological functions
  publication-title: Viruses.
  doi: 10.3390/v14020383
– volume: 32
  start-page: e2381
  year: 2022
  ident: CR5
  article-title: Evolution of the SARS-CoV-2 omicron variants BA.1 to BA.5: Implications for immune escape and transmission
  publication-title: Rev. Med. Virol.
  doi: 10.1002/rmv.2381
– year: 2018
  ident: CR33
  article-title: A single amino acid change in the marburg virus glycoprotein arises during serial cell culture passages and attenuates the virus in a macaque model of disease
  publication-title: mSphere.
  doi: 10.1128/mSphere.00401-17
– ident: CR2
– ident: CR16
– volume: 3
  start-page: 249
  year: 2022
  end-page: 261
  ident: CR19
  article-title: Vaccination-infection interval determines cross-neutralization potency to SARS-CoV-2 Omicron after breakthrough infection by other variants
  publication-title: Medicine
  doi: 10.1016/j.medj.2022.02.006
– volume: 87
  start-page: 12552
  year: 2013
  end-page: 12561
  ident: CR21
  article-title: Middle East respiratory syndrome coronavirus infection mediated by the transmembrane serine protease TMPRSS2
  publication-title: J. Virol.
  doi: 10.1128/JVI.01890-13
– volume: 579
  start-page: 270
  year: 2020
  end-page: 273
  ident: CR1
  article-title: A pneumonia outbreak associated with a new coronavirus of probable bat origin
  publication-title: Nature
  doi: 10.1038/s41586-020-2012-7
– ident: CR6
– year: 2020
  ident: CR26
  article-title: Duration of infectiousness and correlation with RT-PCR cycle threshold values in cases of COVID-19, England, January to May 2020
  publication-title: Euro Surveill.
  doi: 10.2807/1560-7917.ES.2020.25.32.2001483
– volume: 603
  start-page: 679
  year: 2022
  end-page: 686
  ident: CR3
  article-title: Rapid epidemic expansion of the SARS-CoV-2 Omicron variant in southern Africa
  publication-title: Nature
  doi: 10.1038/s41586-022-04411-y
– volume: 11
  start-page: 277
  year: 2022
  end-page: 283
  ident: CR14
  article-title: SARS-CoV-2 Omicron variant shows less efficient replication and fusion activity when compared with Delta variant in TMPRSS2-expressed cells
  publication-title: Emerg. Microbes Infect.
  doi: 10.1080/22221751.2021.2023329
– year: 2024
  ident: CR8
  article-title: Increased faecal shedding in SARS-CoV-2 variants BA.2.86 and JN.1
  publication-title: Lancet Infect. Dis.
  doi: 10.1016/S1473-3099(24)00155-5
– volume: 572
  start-page: 64
  year: 2022
  end-page: 71
  ident: CR15
  article-title: Identification of potential COVID-19 treatment compounds which inhibit SARS Cov2 prototypic, delta and omicron variant infection
  publication-title: Virology
  doi: 10.1016/j.virol.2022.05.004
– year: 2021
  ident: CR13
  article-title: Assessment of SARS-CoV-2 infectivity of upper respiratory specimens from COVID-19 patients by virus isolation using VeroE6/TMPRSS2 cells
  publication-title: BMJ Open Respir. Res.
  doi: 10.1136/bmjresp-2020-000830
– volume: 87
  start-page: 1691
  year: 2006
  end-page: 1695
  ident: CR11
  article-title: Analysis of ACE2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus
  publication-title: J. Gen. Virol.
  doi: 10.1099/vir.0.81749-0
– volume: 74
  start-page: 43
  year: 2023
  end-page: 47
  ident: CR30
  article-title: Investigation of culture cells used for isolation and culture of severe acute respiratory syndrome coronavirus 2, omicron variant, isolated in Tokyo
  publication-title: Ann. Rep. Tokyo Metr. Inst. Public Health
– volume: 382
  start-page: 2081
  year: 2020
  end-page: 2090
  ident: CR25
  article-title: Presymptomatic SARS-CoV-2 infections and transmission in a skilled nursing facility
  publication-title: N. Engl. J. Med.
  doi: 10.1056/NEJMoa2008457
– volume: 387
  start-page: 86
  year: 2022
  end-page: 88
  ident: CR4
  article-title: Neutralization escape by SARS-CoV-2 omicron subvariants BA.2.12.1, BA.4, and BA.5
  publication-title: N. Engl. J. Med.
  doi: 10.1056/NEJMc2206576
– volume: 30
  start-page: 1527
  year: 2022
  end-page: 1539
  ident: CR23
  article-title: Characterization of the enhanced infectivity and antibody evasion of Omicron BA.2.75
  publication-title: Cell Host Microbe
  doi: 10.1016/j.chom.2022.09.018
– volume: 109
  start-page: 2859
  year: 2023
  end-page: 2862
  ident: CR7
  article-title: WHO declares the end of the COVID-19 global health emergency: lessons and recommendations from the perspective of ChatGPT/GPT-4
  publication-title: Int. J. Surg.
  doi: 10.1097/JS9.0000000000000521
– ident: CR17
– volume: 39
  start-page: 1059
  year: 2020
  end-page: 1061
  ident: CR27
  article-title: Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards
  publication-title: Eur. J. Clin. Microbiol. Infect. Dis.
  doi: 10.1007/s10096-020-03913-9
– ident: CR9
– volume: 73
  start-page: 304
  year: 2020
  end-page: 307
  ident: CR10
  article-title: Development of genetic diagnostic methods for detection for novel coronavirus 2019(nCoV-2019) in Japan
  publication-title: Jpn. J. Infect. Dis.
  doi: 10.7883/yoken.JJID.2020.061
– volume: 89
  start-page: 6773
  year: 2015
  end-page: 6781
  ident: CR32
  article-title: Particle-to-PFU ratio of Ebola virus influences disease course and survival in cynomolgus macaques
  publication-title: J Virol
  doi: 10.1128/JVI.00649-15
– volume: 18
  start-page: 16
  year: 2021
  ident: CR20
  article-title: Evaluation of SARS-CoV-2 neutralizing antibodies using a vesicular stomatitis virus possessing SARS-CoV-2 spike protein
  publication-title: Virol. J.
  doi: 10.1186/s12985-021-01490-7
– volume: 117
  start-page: 7001
  year: 2020
  end-page: 7003
  ident: CR12
  article-title: Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.2002589117
– volume: 14
  start-page: 4198
  year: 2023
  ident: CR28
  article-title: Structural delineation and computational design of SARS-CoV-2-neutralizing antibodies against Omicron subvariants
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-023-39890-8
– volume: 26
  start-page: 106634
  year: 2023
  ident: CR31
  article-title: Cell culture systems for isolation of SARS-CoV-2 clinical isolates and generation of recombinant virus
  publication-title: iScience
  doi: 10.1016/j.isci.2023.106634
– volume: 18
  year: 2023
  ident: CR22
  article-title: Structure, dynamics and free energy studies on the effect of point mutations on SARS-CoV-2 spike protein binding with ACE2 receptor
  publication-title: PLoS ONE
  doi: 10.1371/journal.pone.0289432
– volume: 603
  start-page: 706
  year: 2022
  end-page: 714
  ident: CR24
  article-title: Altered TMPRSS2 usage by SARS-CoV-2 Omicron impacts infectivity and fusogenicity
  publication-title: Nature
  doi: 10.1038/s41586-022-04474-x
– volume: 87
  start-page: 12552
  year: 2013
  ident: 75038_CR21
  publication-title: J. Virol.
  doi: 10.1128/JVI.01890-13
– volume: 89
  start-page: 6773
  year: 2015
  ident: 75038_CR32
  publication-title: J Virol
  doi: 10.1128/JVI.00649-15
– ident: 75038_CR9
– volume: 581
  start-page: 465
  year: 2020
  ident: 75038_CR29
  publication-title: Nature
  doi: 10.1038/s41586-020-2196-x
– volume: 3
  start-page: 249
  year: 2022
  ident: 75038_CR19
  publication-title: Medicine
  doi: 10.1016/j.medj.2022.02.006
– volume: 382
  start-page: 2081
  year: 2020
  ident: 75038_CR25
  publication-title: N. Engl. J. Med.
  doi: 10.1056/NEJMoa2008457
– volume: 87
  start-page: 1691
  year: 2006
  ident: 75038_CR11
  publication-title: J. Gen. Virol.
  doi: 10.1099/vir.0.81749-0
– volume: 32
  start-page: e2381
  year: 2022
  ident: 75038_CR5
  publication-title: Rev. Med. Virol.
  doi: 10.1002/rmv.2381
– volume: 572
  start-page: 64
  year: 2022
  ident: 75038_CR15
  publication-title: Virology
  doi: 10.1016/j.virol.2022.05.004
– volume: 117
  start-page: 7001
  year: 2020
  ident: 75038_CR12
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.2002589117
– volume: 74
  start-page: 43
  year: 2023
  ident: 75038_CR30
  publication-title: Ann. Rep. Tokyo Metr. Inst. Public Health
– year: 2022
  ident: 75038_CR34
  publication-title: Viruses.
  doi: 10.3390/v14020383
– ident: 75038_CR2
  doi: 10.1038/s41564-020-0695-z
– volume: 18
  start-page: 16
  year: 2021
  ident: 75038_CR20
  publication-title: Virol. J.
  doi: 10.1186/s12985-021-01490-7
– volume: 30
  start-page: 1527
  year: 2022
  ident: 75038_CR23
  publication-title: Cell Host Microbe
  doi: 10.1016/j.chom.2022.09.018
– volume: 603
  start-page: 679
  year: 2022
  ident: 75038_CR3
  publication-title: Nature
  doi: 10.1038/s41586-022-04411-y
– ident: 75038_CR18
– volume: 11
  start-page: 277
  year: 2022
  ident: 75038_CR14
  publication-title: Emerg. Microbes Infect.
  doi: 10.1080/22221751.2021.2023329
– volume: 603
  start-page: 706
  year: 2022
  ident: 75038_CR24
  publication-title: Nature
  doi: 10.1038/s41586-022-04474-x
– ident: 75038_CR6
– volume: 109
  start-page: 2859
  year: 2023
  ident: 75038_CR7
  publication-title: Int. J. Surg.
  doi: 10.1097/JS9.0000000000000521
– volume: 26
  start-page: 106634
  year: 2023
  ident: 75038_CR31
  publication-title: iScience
  doi: 10.1016/j.isci.2023.106634
– ident: 75038_CR16
  doi: 10.17504/protocols.io.bp2l6n26rgqe/v3
– volume: 579
  start-page: 270
  year: 2020
  ident: 75038_CR1
  publication-title: Nature
  doi: 10.1038/s41586-020-2012-7
– year: 2020
  ident: 75038_CR26
  publication-title: Euro Surveill.
  doi: 10.2807/1560-7917.ES.2020.25.32.2001483
– year: 2024
  ident: 75038_CR8
  publication-title: Lancet Infect. Dis.
  doi: 10.1016/S1473-3099(24)00155-5
– year: 2021
  ident: 75038_CR13
  publication-title: BMJ Open Respir. Res.
  doi: 10.1136/bmjresp-2020-000830
– volume: 39
  start-page: 1059
  year: 2020
  ident: 75038_CR27
  publication-title: Eur. J. Clin. Microbiol. Infect. Dis.
  doi: 10.1007/s10096-020-03913-9
– volume: 18
  year: 2023
  ident: 75038_CR22
  publication-title: PLoS ONE
  doi: 10.1371/journal.pone.0289432
– year: 2018
  ident: 75038_CR33
  publication-title: mSphere.
  doi: 10.1128/mSphere.00401-17
– volume: 387
  start-page: 86
  year: 2022
  ident: 75038_CR4
  publication-title: N. Engl. J. Med.
  doi: 10.1056/NEJMc2206576
– ident: 75038_CR17
  doi: 10.17504/protocols.io.betejeje
– volume: 73
  start-page: 304
  year: 2020
  ident: 75038_CR10
  publication-title: Jpn. J. Infect. Dis.
  doi: 10.7883/yoken.JJID.2020.061
– volume: 14
  start-page: 4198
  year: 2023
  ident: 75038_CR28
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-023-39890-8
SSID ssj0000529419
Score 2.4311464
Snippet The cell culture-based isolation of novel coronavirus SARS-CoV-2 from clinical specimens obtained from patients with suspected COVID-19 is important not only...
Abstract The cell culture-based isolation of novel coronavirus SARS-CoV-2 from clinical specimens obtained from patients with suspected COVID-19 is important...
SourceID doaj
pubmedcentral
proquest
pubmed
crossref
springer
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage 24858
SubjectTerms 631/326/596
631/326/596/4130
ACE2
Angiotensin-converting enzyme 2
Angiotensin-Converting Enzyme 2 - genetics
Angiotensin-Converting Enzyme 2 - metabolism
Animals
Cell culture
Chlorocebus aethiops
Coronaviruses
COVID-19
COVID-19 - metabolism
COVID-19 - virology
Humanities and Social Sciences
Humans
Infectivity
multidisciplinary
Neutralization
SARS-CoV-2
SARS-CoV-2 - genetics
SARS-CoV-2 - isolation & purification
SARS-CoV-2 - metabolism
Science
Science (multidisciplinary)
Serine Endopeptidases - genetics
Serine Endopeptidases - metabolism
Severe acute respiratory syndrome coronavirus 2
Stomatitis
Titration
TMPRSS2
Vero Cells
Viral Load
Virus isolation
SummonAdditionalLinks – databaseName: DOAJ Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1LbxMxELZQpUpcEG8WSmUkbmB1_dq1jyFNVZAKqGmj3iyv14FedlGTSu25f5wZe5M2PMSFa2xpR_PIfON5EfLWg4_nAjOsAl-rYu2ZncfIog3CA7zWtsFu5KPP1eGp-nSmz-6s-sKasDweODNuL-o6etl4ExRXwStrlCyDtsFUygoe8N8XfN6dYCpP9RZWcTt0yZTS7C3AU2E3mVAMU3dA14YnSgP7_4Qyfy-W_CVjmhzRwUPyYECQdJQpf0Tuxe4x2c47Ja-fkJv8TBBbGnE6hA_XtJ_T6eh4ysb9jAl6DtqWxEGxtYSOv8w-7jNu6apHkmLzJc78X1Asiv9GZ_Gin1QUn_gXFCs-41WunoWzk6Ovx9OpoL5raVr3R0fjiXhKTg8mJ-NDNmxaYAGYuGRNaDFU0KW3jQ_GQlilTTAmAICRNUig0rwOulWtUY2MsjF-jm7NGh5iJSv5jGx1fRdfEGrbRkJUjYEiCEtxH9uoAQSZ0gISMaog71Zcdz_yQA2XEuHSuCwjBzJySUYObn9Awaxv4jDs9AOoiBtUxP1LRQqysxKrGyx04SQH6ApoqYZvvFkfg20hN30X-8t8pxYAiOqCPM9asKZEWiUxGiyI2dCPDVI3T7rz72l-N4SgYAWmLMj7lSrd0vV3Xrz8H7x4Re4LtAHwvkLskK3lxWV8DbBq2ewmC_oJXGYaHA
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: Health & Medical Collection
  dbid: 7X7
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfR3LbtQw0IIiJC6ovNMWZCRuYDXxI7FPaNluVZAKqNuu9mY5jrf0kpTNVmrP_DgzTrLVlsc1tpSxZ8bzniHknQMZn3GMsHL0VoXCMbMIgQXjuQP1WpkSq5GPv-ZHZ_LLXM17h1vbp1UOb2J8qKvGo498X2SgaoB0K-THy58Mp0ZhdLUfoXGfPMDWZZjSVcyLtY8Fo1gyM32tTCr0fgvyCmvKuGQYwAPoNuRRbNv_N13zz5TJO3HTKI4Ot8njXo-kow7xT8i9UD8lD7vJkjfPyK_OWRAqGrBHhPM3tFnQ6ehkysbNjHF6ATQXkUKxwISOv80-H7DM0KFSkmIJJnb-bymmxp_TWVg2k5yio7-lmPcZrrscWlg7Pf5-Mp1y6uqKxqF_dDSe8Ofk7HByOj5i_bwF5qXhK1b6Cg0GlTpTOq8NGFdKe609qDGiUEXIVVZ4VclKy1IEUWq3QOFmdOZDLnLxgmzVTR1eEWqqUoBtjeaik4ADF6qgQBXSqQF9RMuEvB9u3V52bTVsDIcLbTscWcCRjTiysPsTIma9E1tixw_N8tz2HGYDAOhE6bSXmfTwVy1F6pXxOofTZT4hewNabc-nrb2lqoS8XS8Dh-Ftujo0V92egoNaVCTkZUcFa0iEkQJtwoToDfrYAHVzpb74Ebt4gyEKvKDThHwYSOkWrn_fxc7_j7FLHnGkbpCunO-RrdXyKrwGtWlVvom88RtKdhHf
  priority: 102
  providerName: ProQuest
– databaseName: Springer Nature OA Free Journals (WRLC)
  dbid: C6C
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Lb9QwELZKERIXVN6hBRmJG0Rs_Ejs4zZsVZAKqNuuerMcZ7btJUG7W6k99493xkkWLZQD13iSTDzjzDeehxn74NHGZ4IirIJ2q6DwqZ0DpGCD8Aivta2oGvnoe354qr6d6bMtJoZamJi0H1taxt_0kB32eYmGhorBhEop8oaPfcAeUut20uoyL9f7KhS5Upnt62OQ8p5bN2xQbNV_H778O03yj1hpNEEHO-xJjx35uOP2KduC5hl71J0mefOc3XYbBFBzoL4QPtzwds6n4-NpWrazVPBL1LMoCE5FJbz8Mfv6Jc0sH6ojOZVdUrf_Jad0-HM-g0U7yTlt7i855XrCdZc3i2MnRz-Pp1PBfVPzeNAfH5cT8YKdHkxOysO0P2MhDcqKVVqFmpwEPfK28sFYdKi0CcYEhC6y0AXkOiuCrlVtVCVBVsbPyaBZkwXIZS5fsu2mbeA147auJPrT5CJ6hTLwUING-GNGFjGIUQn7OMy6-9W10nAxBC6N62TkUEYuysgh9T4JZk1JbbDjhXZx7nq1cIAMell5E1SmAr7VKDkK2gaT49dlIWF7g1hdvzaXTmYIWhEnFfiO9-thXFU0m76B9qqjKQRCoSJhrzotWHMirZLkBybMbOjHBqubI83lRezcjc4n6r8ZJezToEq_-fr3XLz5P_Jd9liQtqOFFWKPba8WV_AWodOqehfXyh2LhA-3
  priority: 102
  providerName: Springer Nature
Title Improved efficacy of SARS-CoV-2 isolation from COVID-19 clinical specimens using VeroE6 cells overexpressing TMPRSS2 and human ACE2
URI https://link.springer.com/article/10.1038/s41598-024-75038-4
https://www.ncbi.nlm.nih.gov/pubmed/39438626
https://www.proquest.com/docview/3119350974
https://www.proquest.com/docview/3119722937
https://pubmed.ncbi.nlm.nih.gov/PMC11496680
https://doaj.org/article/e57ea3ba8c414ca498430c59c864921c
Volume 14
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lj9MwEB7tQ6C9IN4ElspI3CDQ2E5iHxDqhq6WSl1W7bbqLXIcd1kJJdB2pe2ZP85MHkWFcuEUKXZkZx6abzyeGYDXBm18wCnCyum0ysXG13PnfKctNwivQ51RNvLwPDqbyMEsnO1B2-6oIeByp2tH_aQmi2_vbn-sP6LCf6hTxtX7JRohShTj0qeoHC65D4domWJS1GED9-ta31zLQDe5M7s_PYK7QktBQH_LVFUV_XfB0L9vU_4RUq0s1el9uNdATNarZeIB7LniIdypm06uH8HP-hzB5cxR-Qhj16ycs3FvNPaTcupzdo3iWPGLUe4JS75MP3_yA83aJEpG2ZnUFGDJ6Nb8FZu6RdmPGMUAloyuhLrb-notjl0OL0bjMWemyFnVD5D1kj5_DJPT_mVy5jetGHwrNV_5mc3Jlwi7RmfGKo1-V6isUhYRjojD2EVhENswl7mSmXAiU2ZOdk-rwLpIROIJHBRl4Z4B03km0O0mT9JIZIdxuQsRJamuRqiipAdvWqqn3-uKG2kVKRcqrdmVIrvSil0pzj4hxmxmUrXs6kW5uEob5UsdbtCIzCgrA2lxVSVF14baqgj_LrAeHLdsTVsJTEWA2BbhVIxrvNoMo_IRNU3hypt6TswRMcUePK2lYLOTVoo8UFvysbXV7ZHi-mtV4Bt9VFQT1fXgbStKv_f1b1o8__-VXsARJyVAo8z5MRysFjfuJaKtVdaB_XgWd-Cw1xuMB_g86Z9fjPBtEiWd6gSjUynZL2xjKH8
linkProvider Scholars Portal
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3Nb9MwFLdGJwQXxDeBAUaCE0RLbCexDwh1XaeWrWVqu2o34zju2CUZbSfomf-Hv5H38tGpfN12ja3E8fs9v28_Ql4bkPEhwwgrQ2-VS4yvZs75TllmQL2OVIrVyINh3DsRH0-j0y3ys6mFwbTK5kwsD-qssOgj3-UhqBog3RLx4eKrj12jMLratNCoYHHoVt_AZFu87-8Dfd8wdtCddHp-3VXAt0KxpZ_aDNXiKDAqNVYqMCEiaaW0IKx5EiUujsLERpnIpEi546k0MzzClQyti3nM4b03yLbgYMq0yPZed3g8Wnt1MG4mQlVX5wRc7i5AQmIVGxM-hgxhPzYkYNko4G_a7Z9Jmr9FaksBeHCX3Kk1V9quoHaPbLn8PrlZ9bJcPSA_KveEy6jDWymMXdFiRsft0djvFFOf0XNAeQkDiiUttPNp2t_3Q0Wb2kyKRZ_Ya2BBMRn_jE7dvOjGFEMLC4qZpu57lbULY5PB8Wg8ZtTkGS3bDNJ2p8sekpNrocUj0sqL3D0hVGUpB2seDVQjgAbGZS4C5UsGCjQgKTzyttl1fVFd5KHLADyXuqKRBhrpkkYaZu8hYdYz8RLu8kExP9M1T2sHCzQ8NdKKUFj4qhQ8sJGyMoa_C61Hdhqy6vpkWOgrHHvk1XoYeBp30-SuuKzmJAwUscQjjysUrFfCleBohXpEbuBjY6mbI_n5l_LecDB9gftk4JF3DZSu1vXvvXj6_994SW71JoMjfdQfHj4jtxkiHWQ7YzuktZxfuuegtC3TFzWnUPL5upnzFwe-TpQ
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9QwELZKEYhLxZuUAkaCE0Sb2E5iHxBa9qEupaXqtqu9uY7jLb0kZbMV7Jl_xa9jJo-tltet19hKHM83nreHkFcGZHzIMMLK0FvlEuOrmXO-U5YZUK8jlWI18v5BvHsiPk6j6Qb52dbCYFpleyZWB3VWWPSRd3gIqgZIt0R0Zk1axGF_-P7iq48dpDDS2rbTqCGy55bfwHwr3436QOvXjA0Hx71dv-kw4Fuh2MJPbYYqchQYlRorFZgTkbRSWhDcPIkSF0dhYqNMZFKk3PFUmhke50qG1sU85vDeG-RmwqMQeSyZJiv_DkbQRKiaOp2Ay04JshLr2ZjwMXgIO7MmC6uWAX_Tc_9M1_wtZluJwuFdstXosLRbg-4e2XD5fXKr7mq5fEB-1I4Kl1GH91MYu6TFjI67R2O_V0x8Rs8B7xUgKBa30N7nyajvh4q2VZoUyz-x60BJMS3_jE7cvBjEFIMMJcWcU_e9zt-FseP9w6PxmFGTZ7RqOEi7vQF7SE6uhRKPyGZe5O4JoSpLOdj1aKoaATQwLnMRqGEyUKALSeGRN-2u64v6Sg9dheK51DWNNNBIVzTSMPsDEmY1E6_jrh4U8zPdcLd2sEDDUyOtCIWFr0rBAxspK2P4u9B6ZKclq27OiFJfIdojL1fDwN24myZ3xWU9J2GgkiUeeVyjYLUSrgRHe9Qjcg0fa0tdH8nPv1Q3iIMRDHwoA4-8baF0ta5_78X2_3_jBbkNLKk_jQ72npI7DIEOQp6xHbK5mF-6Z6C9LdLnFZtQcnrdfPkLJWZRZA
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Improved+efficacy+of+SARS-CoV-2+isolation+from+COVID-19+clinical+specimens+using+VeroE6+cells+overexpressing+TMPRSS2+and+human+ACE2&rft.jtitle=Scientific+reports&rft.au=Kinoshita%2C+Hitomi&rft.au=Yamamoto%2C+Tsukasa&rft.au=Kuroda%2C+Yudai&rft.au=Inoue%2C+Yusuke&rft.date=2024-10-22&rft.pub=Nature+Publishing+Group+UK&rft.eissn=2045-2322&rft.volume=14&rft_id=info:doi/10.1038%2Fs41598-024-75038-4&rft_id=info%3Apmid%2F39438626&rft.externalDocID=PMC11496680
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2045-2322&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2045-2322&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2045-2322&client=summon