Accounting for intensity variation in image analysis of large-scale multiplexed clinical trial datasets

• Published in: The journal of pathology. Clinical research Vol. 9; no. 6; pp. 449 - 463
• Main Authors: Frei, Anja L, McGuigan, Anthony, Sinha, Ritik Rak, Glaire, Mark A, Jabbar, Faiz, Gneo, Luciana, Tomasevic, Tijana, Harkin, Andrea, Iveson, Tim J, Saunders, Mark, Oein, Karin, Maka, Noori, Pezella, Francesco, Campo, Leticia, Hay, Jennifer, Edwards, Joanne, Sansom, Owen J, Kelly, Caroline, Tomlinson, Ian, Kildal, Wanja, Kerr, Rachel S, Kerr, David J, Danielsen, Håvard E, Domingo, Enric, Church, David N, Koelzer, Viktor H
• Format: Journal Article
• Language: English; Norwegian
• Published: England John Wiley & Sons, Inc 01.11.2023; Wiley
• Subjects: Biomarkers, Tumor - analysis; CD20 antigen; CD4 antigen; CD8 antigen; Clinical trials; Colorectal cancer; Cytokeratin; Datasets; digital pathology; fluorescence microscopy; Foxp3 protein; Humans; image analysis; Image processing; Image Processing, Computer-Assisted - methods; Immunofluorescence; Immunohistochemistry; Lymphocytes; Neoplasms; Original; Patients; Tissue Array Analysis; Variation; United Kingdom > UK; United States > US; digital pathology; fluorescence microscopy; image analysis

Multiplex immunofluorescence (mIF) imaging can provide comprehensive quantitative and spatial information for multiple immune markers for tumour immunoprofiling. However, application at scale to clinical trial samples sourced from multiple institutions is challenging due to pre-analytical heterogene...