Comparative evaluation of cDNA library construction approaches for RNA-Seq analysis from low RNA-content human specimens

• Published in: Journal of microbiological methods Vol. 154; pp. 55 - 62
• Main Authors: Masters, T.L., Hilker, C.A., Jeraldo, P.R., Bhagwate, A.V., Greenwood-Quaintance, K.E., Eckloff, B.W., Chia, N., Hanssen, A.D., Abdel, M.P., Yao, J.Z., Jen, J., Patel, R.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2018
• Subjects: Arthroplasty; bacteria; cDNA libraries; cDNA library preparation; complementary DNA; diagnostic techniques; Gene Expression; Gene Library; Genes, Bacterial - genetics; hosts; Humans; Infection - diagnosis; Infection - genetics; Infection - microbiology; infectious diseases; pathogens; Periprosthetic Fractures - microbiology; Periprosthetic joint infection; Reagent Kits, Diagnostic; RNA; RNA sequencing; RNA, Bacterial - analysis; RNA, Bacterial - isolation & purification; sequence analysis; Sequence Analysis - methods; Sequence Analysis, RNA - methods; Streptococcus sanguis - genetics; Streptococcus sanguis - pathogenicity; Synovial Fluid - microbiology; Transcriptome; transcriptomics; RNA sequencing; Periprosthetic joint infection; Gene expression; cDNA library preparation

With the emergence of RNA sequencing technologies, metatranscriptomic studies are rapidly gaining attention as they simultaneously provide insight into gene expression profiles and therefore disease association pathways of microbial pathogens and their hosts. This approach, therefore, holds promise...