Comparative evaluation of cDNA library construction approaches for RNA-Seq analysis from low RNA-content human specimens - Masters, T.L.; Hilker, C.A.; Jeraldo, P.R.; Bhagwate, A.V.; Greenwood-Quaintance, K.E.; Eckloff, B.W.; Chia, N.; Hanssen, A.D.; Abdel, M.P.; Yao, J.Z.; Jen, J.; Patel, R. (Journal of microbiological methods, 2018)
Keywords: Arthroplasty; bacteria; cDNA libraries; cDNA library preparation; complementary DNA; diagnostic techniques; Gene Expression; Gene Library; Genes, Bacterial - genetics; hosts; Humans; Infection - diagnosis; Infection - genetics; Infection - microbiology; infectious diseases; pathogens; Periprosthetic Fractures - microbiology; Periprosthetic joint infection; Reagent Kits, Diagnostic; RNA; RNA sequencing; RNA, Bacterial - analysis; RNA, Bacterial - isolation & purification; sequence analysis; Sequence Analysis - methods; Sequence Analysis, RNA - methods; Streptococcus sanguis - genetics; Streptococcus sanguis - pathogenicity; Synovial Fluid - microbiology; Transcriptome; transcriptomics; RNA sequencing; Periprosthetic joint infection; Gene expression; cDNA library preparation