Development and validation of UHPLC–MS/MS methods for the quantification of colistin in plasma and dried plasma spots

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 129; pp. 551 - 557
• Main Authors: Cangemi, Giuliana, Barco, Sebastiano, Castagnola, Elio, Tripodi, Gino, Favata, Fabio, D’Avolio, Antonio
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 10.09.2016
• Subjects: Anti-Bacterial Agents - blood; Anti-Bacterial Agents - chemistry; Calibration; Chromatography, High Pressure Liquid - methods; Colistin; Colistin - blood; Colistin - chemistry; Dried Blood Spot Testing - methods; Dried blood spots; Dried plasma spot; Drug Monitoring - methods; Humans; LC–MS; Limit of Detection; Plasma - chemistry; Reproducibility of Results; Tandem Mass Spectrometry - methods; Therapeutic drug monitoring; UHPLC–MS/MS; Therapeutic drug monitoring; UHPLC–MS/MS; Dried plasma spot; Colistin; Dried blood spots; LC–MS

[Display omitted] •A new method to quantify colistin A and B in dried plasma spots (DPS) is reported.•The method was compared to a standard method to quantify colistin in plasma.•Colistin on DPS was found to be stable at least for one week at 20–25°C.•A good correlation was observed among the two ch...