Rapid Microbial Community Changes During Initial Stages of Pine Litter Decomposition

• Published in: Microbial ecology Vol. 77; no. 1; pp. 56 - 75
• Main Authors: Gołębiewski, Marcin, Tarasek, Agata, Sikora, Marcin, Deja-Sikora, Edyta, Tretyn, Andrzej, Niklińska, Maria
• Format: Journal Article
• Language: English
• Published: New York Springer Science + Business Media 01.01.2019; Springer US; Springer Nature B.V
• Subjects: Archaea; Archaea - classification; Archaea - genetics; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - metabolism; Biodiversity; Biogeochemical cycle; Biogeochemical cycles; Biogeochemistry; Biomedical and Life Sciences; Communities; Community structure; Decomposers; Decomposition; Decompression; Deoxyribonucleic acid; DNA; DNA - isolation & purification; DNA, Ribosomal - genetics; Ecology; Ecosystem; Ecosystems; ENVIRONMENTAL MICROBIOLOGY; Eukaryota - classification; Eukaryota - genetics; Eukaryotes; Forests; Fungi; Fungi - classification; Fungi - genetics; Geoecology/Natural Processes; Interactions; Life Sciences; Litter; Metagenomics; Microbial activity; Microbial Ecology; Microbiology; Microbiomes; Microbiota - genetics; Microbiota - physiology; Microorganisms; Nature Conservation; Nematodes; Pine; Pinus - microbiology; RNA, Ribosomal, 16S - genetics; RNA, Ribosomal, 18S - genetics; Soil Microbiology; Trees - microbiology; Water Quality/Water Pollution; Fungal community; Metagenomics; Pine litter decomposition; 16S rDNA pyrosequencing; Bacterial community
• DOI: 10.1007/s00248-018-1209-x
• ISSN: 0095-3628

Plant litter decomposition is a process enabling biogeochemical cycles closing in ecosystems, and decomposition in forests constitutes the largest part of this process taking place in terrestrial biomes. Microbial communities during litter decomposition were studied mainly with low-throughput techniques not allowing detailed insight, particularly into coniferous litter, as it is more difficult to obtain high quality DNA required for analyses. Motivated by these problems, we analyzed archaeal, bacterial, and eukaryotic communities at three decomposition stages: fresh, 3- and 8-month-old litter by 16/18S rDNA pyrosequencing, aiming at detailed insight into early stages of pine litter decomposition. Archaea were absent from our libraries. Bacterial and eukaryotic diversity was greatest in 8-month-old litter and the same applied to bacterial and fungal rDNA content. Community structure was different at various stages of decomposition, and phyllospheric organisms (bacteria: Acetobacteraceae and Pseudomonadaceae members, fungi: Lophodermium, Phoma) were replaced by communities with metabolic capabilities adapted to the particular stage of decomposition. Sphingomonadaceae and Xanthomonadaceae and fungal genera Sistotrema, Ceuthospora, and Athelia were characteristic for 3-month-old samples, while 8-month-old ones were characterized by Bradyrhizobiaceae and nematodes (Plectus). We suggest that bacterial and eukaryotic decomposer communities change at different stages of pine litter decomposition in a way similar to that in broadleaf litter. Interactions between bacteria and eukaryotes appear to be one of the key drivers of microbial community structure.