Functional Characterization of Clostridium difficile Spore Coat Proteins

• Published in: Journal of Bacteriology Vol. 195; no. 7; pp. 1492 - 1503
• Main Authors: Permpoonpattana, Patima, Phetcharaburanin, Jutarop, Mikelsone, Anna, Dembek, Marcin, Tan, Sisareuth, Brisson, Marie-Clémence, La Ragione, Roberto, Brisson, Alain R, Fairweather, Neil, Hong, Huynh A, Cutting, Simon M
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.04.2013
• Subjects: Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; catalase; chitinase; Clostridium difficile; Clostridium difficile - genetics; Clostridium difficile - growth & development; Clostridium difficile - metabolism; Detoxification; Enzymatic activity; enzyme activity; Enzymes - genetics; Enzymes - metabolism; exine; functional properties; Gene Knockout Techniques; Genes; humans; Hydrogen peroxide; insertional mutagenesis; Manganese; Mutagenesis, Insertional; mutants; Mutation; pathogens; peroxiredoxin; Polymerization; post-translational modification; Proteins; spores; Spores, Bacterial - genetics; Spores, Bacterial - growth & development; Spores, Bacterial - metabolism; superoxide dismutase

Spores of Clostridium difficile play a key role in the dissemination of this important human pathogen, and until recently little has been known of their functional characteristics. Genes encoding six spore coat proteins (cotA, cotB, cotCB, cotD, cotE, and sodA) were disrupted by ClosTron insertional...