Acetylcholinesterase of Mammalian Neuromuscular Junctions: Presence of Tailed Asymmetric Acetylcholinesterase in Synaptic Basal Lamina and Sarcolemma

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 80; no. 21; pp. 6698 - 6702
• Main Authors: Dreyfus, Patrick A., Rieger, François, Pinçon-Raymond, Martine
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01.11.1983; National Acad Sciences
• Subjects: acetylcholinesterase; Acetylcholinesterase - metabolism; Animals; Basement membrane; Basement Membrane - enzymology; Cell membranes; Centrifugation; Detergents; Extracellular matrix; Extracellular Matrix - enzymology; Fractionation; Macromolecular Substances; Matrix materials; Mice; microsomes; Motor Endplate - enzymology; Neuromuscular Junction - enzymology; neuromuscular junctions; Salts; Sarcolemma - enzymology; Solubility; Solubilization; Subcellular fractions

A sarcolemma-rich fraction can be isolated after subcellular fractionation of mouse intercostal muscles by sedimentation on a discontinuous sucrose gradient. The quantitative recovery of the acetylcholine receptor in this fraction is about 50%, which indicates the presence of a high proportion of po...