Engineering of acyl ligase domain in non-ribosomal peptide synthetases to change fatty acid moieties of lipopeptides

• Published in: Communications chemistry Vol. 8; no. 1; pp. 17 - 14
• Main Authors: Aoki, Rina, Kumagawa, Eri, Kamata, Kazuaki, Ago, Hideo, Sakai, Naoki, Hasunuma, Tomohisa, Taoka, Naoaki, Ohta, Yukari, Kobayashi, Shingo
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 21.01.2025; Nature Publishing Group; Nature Portfolio
• Subjects: 101/58; 631/45/611; 631/92/60/1167; 639/638/77/603; 639/638/92/349; 82/47; Amino acids; Antimicrobial agents; Binding; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Fatty acids; In vivo methods and tests; Mutation; Peptides

Cyclic lipopeptides (CLPs) produced by the genus Bacillus are amphiphiles composed of hydrophilic amino acid and hydrophobic fatty acid moieties and are biosynthesised by non-ribosomal peptide synthetases (NRPSs). CLPs are produced as a mixture of homologues with different fatty acid moieties, whose...