NaP-TRAP reveals the regulatory grammar in 5’UTR-mediated translation regulation during zebrafish development

• Published in: Nature communications Vol. 15; no. 1; pp. 10898 - 21
• Main Authors: Strayer, Ethan C., Krishna, Srikar, Lee, Haejeong, Vejnar, Charles, Neuenkirchen, Nils, Gupta, Amit, Beaudoin, Jean-Denis, Giraldez, Antonio J.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 30.12.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 49/109; 49/40; 49/47; 49/88; 49/90; 49/91; 5' Untranslated regions; 5' Untranslated Regions - genetics; 631/337/1645/1769; 631/337/1645/2020; Animals; Coding; Control stability; Danio rerio; Embryo, Nonmammalian - metabolism; Embryos; Enhancers; Epitopes; Gene Expression Regulation, Developmental; Humanities and Social Sciences; Humans; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; mRNA stability; multidisciplinary; Peptides; Polyribosomes - metabolism; Protein Biosynthesis; Protein purification; Regulatory sequences; Repressors; Ribosomes - genetics; Ribosomes - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Science; Science (multidisciplinary); Stability; Translation; Zebrafish; Zebrafish - embryology; Zebrafish - genetics; Zebrafish Proteins - genetics; Zebrafish Proteins - metabolism

The cis -regulatory elements encoded in an mRNA determine its stability and translational output. While there has been a considerable effort to understand the factors driving mRNA stability, the regulatory frameworks governing translational control remain more elusive. We have developed a novel mass...