Src Family Kinases Are Involved in the Differential Signaling from Two Splice Forms of c-Kit

• Published in: The Journal of biological chemistry Vol. 278; no. 11; pp. 9159 - 9166
• Main Authors: Voytyuk, Olexandr, Lennartsson, Johan, Mogi, Akira, Caruana, Georgina, Courtneidge, Sara, Ashman, Leonie K, Rönnstrand, Lars
• Format: Journal Article
• Language: English
• Published: United States American Society for Biochemistry and Molecular Biology 14.03.2003
• Subjects: 3T3 Cells; Animals; Blotting, Western; Cell Line; COS Cells; Dose-Response Relationship, Drug; Humans; Indoles - pharmacology; Kinetics; Ligands; Mice; Models, Biological; Phosphorylation; Precipitin Tests; Protein Binding; Protein Isoforms; Protein Structure, Tertiary; Protein-Tyrosine Kinases - metabolism; Proto-Oncogene Proteins c-kit - chemistry; Recombinant Proteins - chemistry; Signal Transduction; src-Family Kinases - antagonists & inhibitors; src-Family Kinases - metabolism; src-Family Kinases - physiology; src-Family Kinases/antagonists & inhibitors/metabolism/physiology; Sulfonamides - pharmacology; Time Factors; Transfection; Western; Drug; Tertiary; Dose-Response Relationship; Models; Biological; Protein Structure; Blotting

In both mice and humans alternate splicing results in isoforms of c-Kit characterized by the presence or the absence of a tetrapeptide sequence, GNNK, in the juxtamembrane region of the extracellular domain. Dramatic differences in the kinetics and magnitude of activation of the intrinsic tyrosine k...