Api m 6: A new bee venom allergen

• Published in: Journal of allergy and clinical immunology Vol. 107; no. 5; pp. 914 - 920
• Main Authors: Kettner, Alexander, Hughes, Graham J., Frutiger, Séverine, Astori, Mireille, Roggero, Mario, Spertini, François, Corradin, Giampietro
• Format: Journal Article Conference Proceeding
• Language: English
• Published: New York, NY Mosby, Inc 01.05.2001; Elsevier
• Subjects: allergens; Allergens - immunology; Allergens - isolation & purification; Allergic diseases; Amino Acid Motifs; Amino Acid Sequence; Animals; Antibody Specificity; Antigens, Plant; Api m 6; Api m 6 antigen; Apis mellifera; bee venom; Bee Venoms - chemistry; Bee Venoms - immunology; Biological and medical sciences; carboxypeptidase; Chromatography, Gel; Chromatography, High Pressure Liquid; Humans; Hymenoptera; Hypersensitivity, Immediate - blood; Hypersensitivity, Immediate - immunology; IgE; Immunoglobulin E - blood; Immunoglobulin E - immunology; Immunopathology; immunotherapy; Insect Proteins - immunology; Insect Proteins - isolation & purification; Lymphocyte Activation; matrix-assisted laser desorption-ionization time of flight mass spectrometry; Medical sciences; Molecular Sequence Data; Protein Denaturation; Skin allergic diseases. Stinging insect allergies; T cells; T-Lymphocytes - immunology; VIT; RT; MALDI-TOF; DTT; IgE; matrix-assisted laser desorption-ionization time of flight mass spectrometry; PLA2; MW; T cells; carboxypeptidase; allergens; BV; Api m 6; SI; bee venom; immunotherapy; Hymenoptera; Human; Immunopathology; Allergy; Immune response; Bee; Insecta; Apis; Apidae; Primary structure; Identification; Cellular immunity; Characterization; Apoidea; Arthropoda; Venom; T-Lymphocyte; Invertebrata; Humoral immunity; Aminoacid sequence; Aculeata; Allergen

Background: Characterization of the primary structure of allergens is a prerequisite for the design of new diagnostic and therapeutic tools for allergic diseases. Objective: The purpose of this study was the identification and characterization of a low-molecular-weight, IgE-binding, bee venom (BV) a...