Uncovering the mechanism of Huangkui capsule in the treatment of diabetic kidney disease based on network pharmacology and experimental validation

• Published in: Scientific reports Vol. 15; no. 1; pp. 6503 - 18
• Main Authors: Liu, Junhong, Li, Ziwei, Zhang, ZongYao, Shen, Zhongyuan
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 22.02.2025; Nature Publishing Group; Nature Portfolio
• Subjects: 692/163; 692/4022/1585; AKT1 protein; Apoptosis; Apoptosis - drug effects; Capsules; Cell Line; Diabetes; Diabetes mellitus; Diabetic kidney disease; Diabetic Nephropathies - drug therapy; Diabetic Nephropathies - genetics; Diabetic Nephropathies - metabolism; Diabetic Nephropathies - pathology; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - pharmacology; Enzyme-linked immunosorbent assay; Experimental validation; Flow cytometry; Genes; Huangkui capsule; Humanities and Social Sciences; Humans; Immunofluorescence; Kidney diseases; Kidneys; Molecular docking; Molecular Docking Simulation; Molecular dynamics; Molecular Dynamics Simulation; Molecular modelling; multidisciplinary; Network Pharmacology; Oxidative stress; Oxidative Stress - drug effects; p53 Protein; Pharmacology; Protein interaction; Protein Interaction Maps - drug effects; Science; Science (multidisciplinary); Experimental validation; Network pharmacology; Huangkui capsule; Molecular docking; Diabetic kidney disease

Diabetic kidney disease (DKD) is a main complication of diabetes mellitus. experimental in vitro validation and Network pharmacology were used in this study to explore the potential mechanism of Huangkui capsules (HKC) in treating DKD. First, we used CCK8 to analyze the optimal drug concentration of...