The challenge of measuring IL-33 in serum using commercial ELISA: lessons from asthma

• Published in: Clinical and experimental allergy Vol. 46; no. 6; pp. 884 - 887
• Main Authors: Ketelaar, M. E., Nawijn, M. C., Shaw, D. E., Koppelman, G. H., Sayers, I.
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.06.2016; Wiley Subscription Services, Inc
• Subjects: Asthma; Asthma - blood; Asthma - diagnosis; Asthma - immunology; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - standards; Health care industry; Humans; IL-1RL1; IL-33; immunoassay; interference; Interleukin-33 - blood; Reagent Kits, Diagnostic - standards; Reproducibility of Results; sensitivity; serum; specificity; Studies; IL-1RL1; specificity; asthma; interference; sensitivity; serum; IL-33; immunoassay
• ISSN: 0954-7894; 1365-2222; 1365-2222
• DOI: 10.1111/cea.12718

Summary Background Interleukin‐33 (IL‐33) has been subject of extensive study in the context of inflammatory disorders, particularly in asthma. Many human biological samples, including serum, have been used to determine the protein levels of IL‐33, aiming to investigate its involvement in asthma. Re...