Shortened microsatellite d(CA)21 sequence down-regulates promoter activity of matrix metalloproteinase 9 gene

• Published in: FEBS letters Vol. 455; no. 1; pp. 70 - 74
• Main Authors: Shimajiri, Shohei, Arima, Nobuyuki, Tanimoto, Akihide, Murata, Yoshitake, Hamada, Tetsuo, Wang, Ke-Yong, Sasaguri, Yasuyuki
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 16.07.1999
• Subjects: Base Sequence; Cell Nucleus - metabolism; Collagenases - genetics; d(CA) repeat; DMEM, Dulbecco's modified Eagle's medium; DNA; Down-Regulation; EGF, epidermal growth factor; EMSA, electrophoretic mobility shift assay; Esophageal cancer; FCS, fetal calf serum; Gene transcription; Luciferases - genetics; Matrix Metalloproteinase 9; Microsatellite; Microsatellite Repeats - genetics; MMP-9, matrix metalloproteinase 9; Molecular Sequence Data; PCR, polymerase chain reaction; Promoter Regions, Genetic; Tumor Cells, Cultured; Microsatellite; Matrix metalloproteinase 9; DMEM, Dulbecco’s modified Eagle’s medium; EGF, epidermal growth factor; d(CA) repeat; PCR, polymerase chain reaction; EMSA, electrophoretic mobility shift assay; Esophageal cancer; MMP-9, matrix metalloproteinase 9; Gene transcription; FCS, fetal calf serum

One characteristic elements in the promoter of the matrix metalloproteinase 9 (MMP-9) gene is the d(CA) repeat. To investigate whether this element regulates the transcription of the MMP-9 gene and its enzymatic activities, we sequenced the promoter region isolated from esophageal carcinoma cell lin...