Cloning and expression of a rat acetylcholinesterase subunit: generation of multiple molecular forms and complementarity with a Torpedo collagenic subunit

• Published in: Journal of neurochemistry Vol. 60; no. 1; p. 337
• Main Authors: Legay, C, Bon, S, Vernier, P, Coussen, F, Massoulié, J
• Format: Journal Article
• Language: English
• Published: England 01.01.1993
• Subjects: Acetylcholinesterase - chemistry; Acetylcholinesterase - genetics; Acetylcholinesterase - metabolism; Amino Acid Sequence; Animals; Base Sequence; Blotting, Northern; Brain - metabolism; Cell Line, Transformed; Central Nervous System - metabolism; Cloning, Molecular; Collagen - metabolism; DNA - genetics; Mice; Molecular Conformation; Molecular Sequence Data; Rats; RNA, Messenger - metabolism; Torpedo; Transfection

We obtained a cDNA clone encoding one type of catalytic subunit of acetylcholinesterase (AChE) from rat brain (T subunit). The coding sequence shows a high frequency of (G+C) at the third position of the codons (66%), as already noted for several AChEs, in contrast with mammalian butyrylcholinestera...