MHC Class II levels and intracellular localization in human dendritic cells are regulated by calmodulin kinase II

• Published in: Journal of leukocyte biology Vol. 82; no. 3; pp. 686 - 699
• Main Authors: Herrmann, Tara L., Agrawal, Reitu S., Connolly, Sean F., McCaffrey, Ramona L., Schlomann, Jamie, Kusner, David J.
• Format: Journal Article
• Language: English
• Published: United States Society for Leukocyte Biology 01.09.2007
• Subjects: Adult; antigen presentation/processing; Calcium-Calmodulin-Dependent Protein Kinase Type 2; Calcium-Calmodulin-Dependent Protein Kinases - antagonists & inhibitors; Calcium-Calmodulin-Dependent Protein Kinases - genetics; Calcium-Calmodulin-Dependent Protein Kinases - metabolism; Caspase 3 - metabolism; Cathepsin D - metabolism; cell activation; Cells, Cultured; Cysteine Endopeptidases - metabolism; Dendritic Cells - metabolism; Flow Cytometry; Histocompatibility Antigens Class II - genetics; Histocompatibility Antigens Class II - metabolism; Humans; Immunoblotting; Lysosomes - metabolism; Membrane Proteins - metabolism; Monocytes; Protein Processing, Post-Translational; RNA Processing, Post-Transcriptional; RNA Stability; Signal Transduction; Subcellular Fractions; transcription factors

Dendritic cells (DC) are professional APC, which activate the adaptive immune response. A Ca2+‐calmodulin (CaM)‐CaM kinase II (CaMKII) pathway regulates maturation and MHC Class II antigen presentation in human DC. The objective of this study was to characterize the mechanisms by which CaMKII modula...