Neuroprotective effects of PDGF against oxidative stress and the signaling pathway involved

• Published in: Journal of neuroscience research Vol. 88; no. 6; pp. 1273 - 1284
• Main Authors: Zheng, Lianshun, Ishii, Yoko, Tokunaga, Ayano, Hamashima, Takeru, Shen, Jie, Zhao, Qing-Li, Ishizawa, Shin, Fujimori, Toshihiko, Nabeshima, Yo-ichi, Mori, Hisashi, Kondo, Takashi, Sasahara, Masakiyo
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.05.2010
• Subjects: Animals; Apoptosis - physiology; Caspase 3 - metabolism; Cell Survival - physiology; Cells, Cultured; Cerebral Cortex - enzymology; Cerebral Cortex - physiology; Humans; Hydrogen Peroxide - metabolism; Hydrogen Peroxide - toxicity; MAP kinase; Mice; Mice, Inbred C57BL; Mice, Transgenic; neuro-protection; Neurons - enzymology; Neurons - physiology; oxidative stress; Oxidative Stress - physiology; PI3-K/Akt kinase; platelet-derived growth factor; Platelet-Derived Growth Factor - metabolism; Proto-Oncogene Proteins c-akt - metabolism; Proto-Oncogene Proteins c-sis; Receptor, Platelet-Derived Growth Factor beta - deficiency; Receptor, Platelet-Derived Growth Factor beta - genetics; Receptor, Platelet-Derived Growth Factor beta - metabolism; Recombinant Proteins - metabolism; Signal Transduction
• ISSN: 0360-4012; 1097-4547; 1097-4547
• DOI: 10.1002/jnr.22302

The neuroprotective effects of platelet‐derived growth factor (PDGF) and the major signaling pathways involved in these were examined using primary cultured mouse cortical neurons subjected to H2O2‐induced oxidative stress. The specific function of the PDGF β‐receptor (PDGFR‐β) was examined by the selective deletion of the corresponding gene using the Cre‐loxP system in vitro. In wild‐type neurons, PDGF‐BB enhanced the survival of these neurons and suppressed H2O2‐induced caspase‐3 activation. The prosurvival effect of PDGF‐AA was less than that of PDGF‐BB. PDGF‐BB highly activated Akt, extracellular signal‐regulated kinase (ERK), c‐jun amino‐terminal kinase (JNK) and p38. PDGF‐AA activated these molecules at lesser extent than PDGF‐BB. In particular, PDGF‐AA induced activation of Akt was at very low level. The neuroprotective effects of PDGF‐BB were antagonized by inhibitors of phosphatidylinositol 3‐kinase (PI3‐K), mitogen‐activated protein kinase kinase (MEK), JNK and p38. The PDGFR‐β‐depleted neurons showed increased vulnerability to oxidative stress, and less responsiveness to PDGF‐BB‐induced cytoprotection and signal activation, in which Akt activation was most strongly suppressed. After all, these results demonstrated the neuroprotective effects of PDGF and the signaling pathways involved against oxidative stress. The effects of PDGF‐BB were more potent than those of PDGF‐AA. This might be due to the activation and additive effects of two PDGFRs after PDGF‐BB stimulation. Furthermore, the PI3‐K/Akt pathway that was deduced to be preferentially activated by PDGFR‐β may explain the potent effects of PDGF‐BB. © 2009 Wiley‐Liss, Inc.