MicroRNA expression profiling of NGF-treated PC12 cells revealed a critical role for miR-221 in neuronal differentiation

• Published in: Neurochemistry international Vol. 60; no. 8; pp. 743 - 750
• Main Authors: Hamada, Nanako, Fujita, Yasunori, Kojima, Toshio, Kitamoto, Aya, Akao, Yukihiro, Nozawa, Yoshinori, Ito, Masafumi
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.06.2012; Elsevier
• Subjects: Animals; Apaf-1 protein; Apoptosis; Axonogenesis; Biological and medical sciences; Blotting, Western; Cell Differentiation - genetics; Differentiation; FOXO3 protein; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; MicroRNA microarray; MicroRNAs - genetics; MicroRNAs - physiology; miR-221; miRNA; mRNA; Nerve growth factor; Nerve Growth Factor - pharmacology; Neuronal differentiation; Neurons - cytology; Neurons - drug effects; non-coding RNA; PC12 Cells; Pheochromocytoma cells; Polymerase chain reaction; Rats; Reverse Transcriptase Polymerase Chain Reaction; Synapsin I; Synaptogenesis; Translation; Vertebrates: nervous system and sense organs; AD; 3′-UTR; Foxo3a; Nerve growth factor; HS; KSR; NGF; MAPK; PC12 cells; GEO; DMEM; FBS; miR-221; Neuronal differentiation; miRNA; MicroRNA microarray; Apaf-1; Rat; Rodentia; Micro RNA; In vitro; Gene silencing; Vertebrata; Mammalia; Cell line; Neuron; Animal; Differentiation

► We identified NGF-regulated miRNAs in PC12 cells by microarray analysis. ► miR-221 overexpression induced and enhanced neuronal differentiation. ► Knockdown of miR-221 attenuated NGF-induced neuronal differentiation. ► miR-221 overexpression decreased protein expression of Foxo3a and Apaf-1. ► miR...