Simultaneous quantification of crocetin esters and picrocrocin changes in Chinese saffron by high-performance liquid chromatography-diode array detector during 15 years of storage
Saffron, which is made up of the dried stigmas of Crocus sativus L., has been successfully cultivated in China since 1970s and Zhejiang province is now the largest producing area in China, but the contents of crocetin esters and picrocrocin in saffron from Zhejiang province has not been determined s...
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Published in | Pharmacognosy Magazine Vol. 11; no. 43; pp. 540 - 545 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
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01.07.2015
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Abstract | Saffron, which is made up of the dried stigmas of Crocus sativus L., has been successfully cultivated in China since 1970s and Zhejiang province is now the largest producing area in China, but the contents of crocetin esters and picrocrocin in saffron from Zhejiang province has not been determined simultaneously by high-performance liquid chromatography (HPLC) and changes of these constituents in Chinese saffron during storage for years has not been studied.
To establish a simple method quantification of the five main compounds including picrocrocin and four crocetin esters in saffron from main producing areas of China and study the influence of storage time on the changes of saffron constituents.
A simple, sensitive, and accurate HPLC method was developed for simultaneous determination of five major active components in saffron and eight samples which collected from the same farm of Zhejiang province in different years were analyzed.
The correlation coefficient values (R (2) > 0.9997) indicated good correlations between the investigated compounds' concentrations and their peak areas within the test ranges. The limits of quantification and detection of the five compounds were 0.53-2.76 μg/mL and 0.11-0.77 μg/mL, respectively. The recoveries ranged from 94.67% to 101.31%, and the overall relative standard deviations for intra-day and inter-day were lower than 3.49%. The method was applied to study the changes of crocetin esters and picrocrocin contents in saffron samples during 15 years of storage. The losses of crocetin esters and picrocrocin in saffron with 1 -year storage were 52.2% and 54.3%, respectively. The trend then declined during subsequent storage.
The developed method can be applied to the intrinsic quality control of saffron. |
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AbstractList | BACKGROUNDSaffron, which is made up of the dried stigmas of Crocus sativus L., has been successfully cultivated in China since 1970s and Zhejiang province is now the largest producing area in China, but the contents of crocetin esters and picrocrocin in saffron from Zhejiang province has not been determined simultaneously by high-performance liquid chromatography (HPLC) and changes of these constituents in Chinese saffron during storage for years has not been studied. OBJECTTo establish a simple method quantification of the five main compounds including picrocrocin and four crocetin esters in saffron from main producing areas of China and study the influence of storage time on the changes of saffron constituents. MATERIALS AND METHODSA simple, sensitive, and accurate HPLC method was developed for simultaneous determination of five major active components in saffron and eight samples which collected from the same farm of Zhejiang province in different years were analyzed. RESULTSThe correlation coefficient values (R (2) > 0.9997) indicated good correlations between the investigated compounds' concentrations and their peak areas within the test ranges. The limits of quantification and detection of the five compounds were 0.53-2.76 μg/mL and 0.11-0.77 μg/mL, respectively. The recoveries ranged from 94.67% to 101.31%, and the overall relative standard deviations for intra-day and inter-day were lower than 3.49%. The method was applied to study the changes of crocetin esters and picrocrocin contents in saffron samples during 15 years of storage. The losses of crocetin esters and picrocrocin in saffron with 1 -year storage were 52.2% and 54.3%, respectively. The trend then declined during subsequent storage. CONCLUSIONThe developed method can be applied to the intrinsic quality control of saffron. Background: Saffron, which is made up of the dried stigmas of Crocus sativus L., has been successfully cultivated in China since 1970s and Zhejiang province is now the largest producing area in China, but the contents of crocetin esters and picrocrocin in saffron from Zhejiang province has not been determined simultaneously by high performance liquid chromatography (HPLC) and changes of these constituents in Chinese saffron during storage for years has not been studied. Object: To establish a simple method quantification of the five main compounds including picrocrocin and four crocetin esters in saffron from main producing areas of China and study the influence of storage time on the changes of saffron constituents. Materials and Methods: A simple, sensitive, and accurate HPLC method was developed for simultaneous determination of five major active components in saffron and eight samples which collected from the same farm of Zhejiang province in different years were analyzed. Results: The correlation coefficient values (R2 > 0.9997) indicated good correlations between the investigated compounds' concentrations and their peak areas within the test ranges. The limits of quantification and detection of the five compounds were 0.53-2.76 μg/mL and 0.11-0.77 μg/mL, respectively. The recoveries ranged from 94.67% to 101.31%, and the overall relative standard deviations for intra day and inter day were lower than 3.49%.The method was applied to study the changes of crocetin esters and picrocrocin contents in saffron samples during 15 years of storage. The losses of crocetin esters and picrocrocin in saffron with 1 year storage were 52.2% and 54.3%, respectively. The trend then declined during subsequent storage. Conclusion: The developed method can be applied to the intrinsic quality control of saffron. Saffron, which is made up of the dried stigmas of Crocus sativus L., has been successfully cultivated in China since 1970s and Zhejiang province is now the largest producing area in China, but the contents of crocetin esters and picrocrocin in saffron from Zhejiang province has not been determined simultaneously by high-performance liquid chromatography (HPLC) and changes of these constituents in Chinese saffron during storage for years has not been studied. To establish a simple method quantification of the five main compounds including picrocrocin and four crocetin esters in saffron from main producing areas of China and study the influence of storage time on the changes of saffron constituents. A simple, sensitive, and accurate HPLC method was developed for simultaneous determination of five major active components in saffron and eight samples which collected from the same farm of Zhejiang province in different years were analyzed. The correlation coefficient values (R (2) > 0.9997) indicated good correlations between the investigated compounds' concentrations and their peak areas within the test ranges. The limits of quantification and detection of the five compounds were 0.53-2.76 μg/mL and 0.11-0.77 μg/mL, respectively. The recoveries ranged from 94.67% to 101.31%, and the overall relative standard deviations for intra-day and inter-day were lower than 3.49%. The method was applied to study the changes of crocetin esters and picrocrocin contents in saffron samples during 15 years of storage. The losses of crocetin esters and picrocrocin in saffron with 1 -year storage were 52.2% and 54.3%, respectively. The trend then declined during subsequent storage. The developed method can be applied to the intrinsic quality control of saffron. |
Audience | Academic |
Author | Yan, Yongqiu Zhu, Xingyi Liu, Ruoxi Tong, Yingpeng Gong, Feng Zhang, Ling Wang, Ping |
AuthorAffiliation | 1 Department of Biochemistry and Molecular Biology, The University of Miami Leonard of Miami Miller School of Medicine, Miami, FL, USA Key Laboratory of Pharmaceutical Engineering of Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, China |
AuthorAffiliation_xml | – name: Key Laboratory of Pharmaceutical Engineering of Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, China – name: 1 Department of Biochemistry and Molecular Biology, The University of Miami Leonard of Miami Miller School of Medicine, Miami, FL, USA |
Author_xml | – sequence: 1 givenname: Yingpeng surname: Tong fullname: Tong, Yingpeng organization: Key Laboratory of Pharmaceutical Engineering of Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, China – sequence: 2 givenname: Yongqiu surname: Yan fullname: Yan, Yongqiu organization: Key Laboratory of Pharmaceutical Engineering of Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, China – sequence: 3 givenname: Xingyi surname: Zhu fullname: Zhu, Xingyi organization: Key Laboratory of Pharmaceutical Engineering of Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, China – sequence: 4 givenname: Ruoxi surname: Liu fullname: Liu, Ruoxi organization: Key Laboratory of Pharmaceutical Engineering of Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, China – sequence: 5 givenname: Feng surname: Gong fullname: Gong, Feng organization: Department of Biochemistry and Molecular Biology, The University of Miami Leonard of Miami Miller School of Medicine, Miami, FL, USA – sequence: 6 givenname: Ling surname: Zhang fullname: Zhang, Ling organization: Department of Biochemistry and Molecular Biology, The University of Miami Leonard of Miami Miller School of Medicine, Miami, FL, USA – sequence: 7 givenname: Ping surname: Wang fullname: Wang, Ping organization: Key Laboratory of Pharmaceutical Engineering of Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, China |
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Keywords | Crocetin esters saffron high-performance liquid chromatography-diode array detector storage picrocrocin |
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Snippet | Saffron, which is made up of the dried stigmas of Crocus sativus L., has been successfully cultivated in China since 1970s and Zhejiang province is now the... Background: Saffron, which is made up of the dried stigmas of Crocus sativus L., has been successfully cultivated in China since 1970s and Zhejiang province is... BACKGROUNDSaffron, which is made up of the dried stigmas of Crocus sativus L., has been successfully cultivated in China since 1970s and Zhejiang province is... |
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SubjectTerms | Accuracy Calibration Chinese medicine Chromatography Health aspects High performance liquid chromatography Original Pharmacological research Quality Saffron (Spice) Sensors Spectrum analysis Traditional Chinese medicine Ultrasonic imaging |
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Title | Simultaneous quantification of crocetin esters and picrocrocin changes in Chinese saffron by high-performance liquid chromatography-diode array detector during 15 years of storage |
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