Assessment of electrophile damage in a human brain endothelial cell line utilizing a clickable alkyne analog of 2-chlorohexadecanal

• Published in: Free radical biology & medicine Vol. 90; pp. 59 - 74
• Main Authors: Nusshold, Christoph, Üllen, Andreas, Kogelnik, Nora, Bernhart, Eva, Reicher, Helga, Plastira, Ioanna, Glasnov, Toma, Zangger, Klaus, Rechberger, Gerald, Kollroser, Manfred, Fauler, Günter, Wolinski, Heimo, Weksler, Babette B., Romero, Ignacio A., Kohlwein, Sepp D., Couraud, Pierre-Olivier, Malle, Ernst, Sattler, Wolfgang
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2016
• Subjects: Aldehydes - metabolism; Alkylation; Alkynes - metabolism; Blood–brain barrier dysfunction; Brain - metabolism; Cells, Cultured; Click chemistry; Endothelial Cells - metabolism; Female; Humans; Hypochlorite; Myeloperoxidase; Proteins - metabolism; Myeloperoxidase; Blood–brain barrier dysfunction; Click chemistry; Hypochlorite

Peripheral leukocytes aggravate brain damage by releasing cytotoxic mediators that compromise blood–brain barrier function. One of the oxidants released by activated leukocytes is hypochlorous acid (HOCl) that is formed via the myeloperoxidase–H2O2–chloride system. The reaction of HOCl with the endo...