Characterization of cationic polymers by asymmetric flow field-flow fractionation and multi-angle light scattering—A comparison with traditional techniques

• Published in: Journal of Chromatography A Vol. 1325; pp. 195 - 203
• Main Authors: Wagner, Michael, Pietsch, Christian, Tauhardt, Lutz, Schallon, Anja, Schubert, Ulrich S.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 17.01.2014; Elsevier
• Subjects: Amines; Analytical chemistry; Applied sciences; Backbone; buffers; Carriers; Cationic; Cationic polymers; Cations - chemistry; Chains (polymeric); Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, Gel - instrumentation; electrolytes; Exact sciences and technology; Field flow fractionation; Fractionation; Fractionation, Field Flow - instrumentation; Fractionation, Field Flow - methods; gel chromatography; Gene delivery; gene transfer; lysine; Magnetic Resonance Spectroscopy - instrumentation; mass spectrometry; Molecular Weight; nanomedicine; nuclear magnetic resonance spectroscopy; Organic polymers; Other chromatographic methods; PDMAEMA; PEI; Physicochemistry of polymers; PLL; Polymers; Polymers - chemistry; Properties and characterization; secondary amines; Structure, morphology and analysis; tertiary amines; ultracentrifugation; Field flow fractionation; Gene delivery; PLL; Cationic polymers; PEI; PDMAEMA; Methacrylate polymer; Molecular weight distribution; Molecular weight determination; Light scattering; Lysine polymer; Polyaminoacid; Multiple scattering; Characterization; Polyelectrolyte; Amine polymer; Gene therapy; Comparative study; Polyethylene imine

•Cationic polymers were characterized by asymmetric flow field-flow fractionation.•Different membranes and eluents were evaluated to find optimal separation conditions.•Analytical ultracentrifugation, DLS, SEC and 1H NMR spectroscopy were used to evaluate the results.•Asymmetric flow field-flow frac...