A liquid chromatography-tandem mass spectrometry-based method for the simultaneous determination of hydroxy sterols and bile acids - John, Clara; Werner, Philipp; Worthmann, Anna; Wegner, Katrin; Tödter, Klaus; Scheja, Ludger; Rohn, Sascha; Heeren, Joerg; Fischer, Markus (Journal of Chromatography A, 2014)
Keywords: adipose tissue; Analytical, structural and metabolic biochemistry; Animals; Bile acids; Bile Acids and Salts - analysis; Biological and medical sciences; blood plasma; Body Fluids - chemistry; Calibration; cholesterol; Chromatography, High Pressure Liquid - methods; derivatization; diet; energy; feces; Feces - chemistry; Fundamental and applied biological sciences. Psychology; gall bladder; HPLC-ESI-QqQ-MS/MS; Hydroxy sterols; inflammation; Investigative techniques, diagnostic techniques (general aspects); Limit of Detection; liquid chromatography; liver; Liver - chemistry; Male; Medical sciences; Mice; Mice, Inbred C57BL; Miscellaneous. Technology; Other biological molecules; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Polar embedded stationary phase; Solid Phase Extraction; Sterol profiling in biological matrices lipid metabolism; Sterols - analysis; tandem mass spectrometry; Tandem Mass Spectrometry - methods; taurine; Terpenes, steroids. Hormones; urine; Bile acids; HPLC-ESI-QqQ-MS/MS; Hydroxy sterols; Polar embedded stationary phase; Sterol profiling in biological matrices lipid metabolism; Steroid; Solid phase extraction; Biological fluid; Tandem mass spectrometry; Chemical analysis; Adipose tissue; Liver; HPLC chromatography; Lipids; Electrospray; Biological material; Chemical enrichment; Tissue; Sample preparation; Bile acid; Clinical biology; Feces; Quantitative analysis; Human; Validation; Healthy subject; Coupled method; Rodentia; Metabolism; Vertebrata; Mammalia; Position isomer; Mouse; Animal; Simultaneous measurement; Sterol