Purified human serum PON1 does not protect LDL against oxidation in the in vitro assays initiated with copper or AAPH

• Published in: Journal of lipid research Vol. 45; no. 12; pp. 2260 - 2268
• Main Authors: Teiber, John F., Draganov, Dragomir I., La Du, Bert N.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2004; Elsevier
• Subjects: 2,2′-azobis-2-amidinopropane hydrochloride; Amidines - metabolism; antioxidants; Aryldialkylphosphatase - isolation & purification; Aryldialkylphosphatase - metabolism; atherosclerosis; Cholesterol, LDL - metabolism; Chromatography, DEAE-Cellulose; Copper - metabolism; Detergents - metabolism; Humans; Oxidation-Reduction; oxidized low density lipoprotein; paraoxonase 1; Phosphatidylcholines - metabolism; paraoxonase 1; atherosclerosis; oxidized low density lipoprotein; antioxidants; 2,2′-azobis-2-amidinopropane hydrochloride

Purified serum paraoxonase (PON1) had been shown to attenuate the oxidation of LDL in vitro. We critically reevaluated the antioxidant properties of serum PON1 in the in vitro assays initiated with copper or the free radical generator 2,2′-azobis-2-amidinopropane hydrochloride (AAPH). The antioxidan...