Microextraction by packed sorbent and high performance liquid chromatography determination of seven non-steroidal anti-inflammatory drugs in human plasma and urine

• Published in: Journal of Chromatography A Vol. 1367; pp. 1 - 8
• Main Authors: Locatelli, Marcello, Ferrone, Vincenzo, Cifelli, Roberta, Barbacane, Renato Carmine, Carlucci, Giuseppe
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 07.11.2014; Elsevier
• Subjects: Analysis; Analytical chemistry; Anti-Inflammatory Agents, Non-Steroidal - blood; Anti-Inflammatory Agents, Non-Steroidal - urine; Biological and medical sciences; Calibration; Chromatography, High Pressure Liquid - methods; General pharmacology; High performance liquid chromatography; Human; Human plasma; Human urine; Humans; Ibuprofen; indomethacin; ketoprofen; Limit of Detection; Linearity; Medical sciences; MEPS-HPLC-PDA; Method development; microextraction; Nonsteroidal antiinflammatory drugs; NSAIDs; particle size; Pharmacology. Drug treatments; Sample preparation; Solid Phase Microextraction - methods; sorbents; Urine; Human plasma; Sample preparation; Method development; NSAIDs; Human urine; MEPS-HPLC-PDA; Biological fluid; Chemical analysis; HPLC chromatography; Blood; Chemical enrichment; Blood plasma; Diode array; Quantitative analysis; Trace analysis; Human; Urine; Validation; Fluorescence detector; Elution; Gradient; Healthy subject; Elimination; Non steroidal antiinflammatory agent; Solid phase microextraction; Reversed phase chromatography; Ultraviolet detector; Syringe

•First MEPS-HPLC–PDA assay for NSAIDs analysis in plasma and urine.•Simultaneous determination of seven NSAIDs in biological matrices.•The method can be adopted for rapid multi-components analyses in biological matrix.•Validation procedure fulfilling ICH Q2(R1) guidelines. This paper reports a new M...